用彗星实验和微核实验检测地面水污染的遗传毒性效应  

Detecting of genotoxic effect for surface-water pollution with comet test and micronucleus test

作　　者：齐宝宁[1] 唐国慧[1] 李劲松 易建华[1] 郭剑锋[1] 苗江丽[1] 

机构地区：[1]西安交通大学医学院劳动卫生与环境卫生教研室,陕西西安710061 [2]西安市卫生监督所,陕西西安710054

出　　处：《中国工业医学杂志》2006年第3期163-165,共3页Chinese Journal of Industrial Medicine

摘　　要：目的探索应用彗星实验(单细胞凝胶电泳技术,SCGE)测定鲤鱼红细胞DNA损伤以评价地面水污染遗传毒性效应的可行性。方法应用单细胞凝胶电泳和微核实验对污染指数(PI)不同的5种河流中采集的鲤鱼的红细胞DNA损伤和微核率进行测定。结果5种地面水水源中的鲤鱼红细胞DNA损伤情况不全相同,与阴性对照组比较差异有显著性(P<0.05),与污染严重程度呈正相关,趋势与微核实验一致。结论SCGE测定鲤鱼红细胞DNA损伤,可以检测地面水的污染状况。Objective To explore the feasibility of assessing the genotoxic effect for surface-water pollution using carp erythrocytes with comet test. Method Measure the incidences of DNA damage and microneucleus in erythrocytes of cyprinoids which were respectively taken from five rivers with different pollution index （PI） by single cell gel electrophoresis （SCGE, or comet test） and micronucleus test. Result Comet test showed that all the carp RBCs had DNA damage that significantly differed from the controls （ P 〈 0.05）, there was obvious positive correlation between severity and pollution level, and quite consistent trend showed in micronucleus test. Conclusion The results suggested that detecting the DNA damage of carp RBCs with comet test might reflect the pollution level of surface-water.

关 键 词：单细胞凝胶电泳(彗星实验) DNA损伤 微核实验 红细胞 

分 类 号：R123.2[医药卫生—环境卫生学]