溶出伏安法对维生素K_3的研究与测定  

STUDY ON THE STRIPPING VOLTAMMETRY AND DETERMINATION OF VITAMIN K_3

作　　者：毕淑云[1] 张敬东[2] 刘英红[1] 王桂芬[1] 

机构地区：[1]延边大学理工学院化学系,延吉133002 [2]延边大学分析测试中心

出　　处：《理化检验（化学分册）》2006年第6期455-456,463,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：应用微分脉冲溶出伏安法（DPSV）对维生素K3在玻碳电极（GCE）上的电化学行为进行了研究。在氨水-NH4Cl（pH9．0）底液中，对维生素K3进行循环伏安扫描，发现有一对良好的氧化还原峰出现，对电极反应机理进行了探讨。阴极溶出伏安峰电流与其浓度在2．0×10^-6～1．0×10^-4mol·L^-1范围内呈线性关系（r=0．9985），检出限为4．0×10^-7mol·L^-1。对于1．0×10^-5mol·L^-1的维生素K3，平行7次测定结果的RSD为1．15％。应用此方法对亚硫酸氢钠甲萘醌注射液进行了测定，方法的回收率为97．3％～102．5％。Differential pulse stripping voltammetric behavior of vitamin K3 at the glassy carbon electrode （GCE） was studied in this paper. In a medium of ammoniacal buffer （pH 9. 0）, a pair of anodic-cathodic peak of vitamin K3 appeared in the cyclic voltammetric scanning. Mechanism of the electrode reaction was discussed. A linear relationship between the cathodic stripping peak current and VK3 concentration in range of 2. 0 × 10^-6 ～1.0 × 10^-4mol · L^-1 was held （r=0. 998 5）, with a detection limit of 4. 0× 10^-7mol · L^-1. RSD was 1.15% for the determination of VIQ at level of 1.0× 10^-5mol · L^-1（n=7）. Recovery of the method was 97. 3%-102. 5%. The method has been applied to the determination of VK3 in medical injection.

关 键 词：维生素K3 玻碳电极 微分脉冲溶出伏安法 注射液 

分 类 号：O657.14[理学—分析化学]