鹿茸多肽的生物学活性研究  

Studies on Biological Activities of Velvet Antler Polypeptides

作　　者：郝林琳[1] 刘松财[1] 夏青娟[2] 章倩倩[1] 侯锋[1] 张永亮[1] 

机构地区：[1]吉林大学农学部畜牧兽医学院,长春130062 [2]吉林农业大学动物科技学院,长春130118

出　　处：《吉林农业大学学报》2006年第3期285-288,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金资助项目(30270973)

摘　　要：采用醇沉法从新鲜马鹿茸中提取出活性多肽。该多肽为一系列分子量低于17kD的多肽混合物，Western blotting检测到其含有胰岛素样生长因子-1（IGF-1）。Bradford法定量总多肽约为1～2mg／g鲜重，RIA法定量IGF-1为300～400pg／g鲜重。小鼠腹腔连续注射多肽6d后，对其促生长、免疫调节、抗氧化、抗疲劳等生物学活性进行了研究。结果表明：小鼠体重较对照组有增加趋势（P〉0．05）；腹腔注射1，4，8mg／kg鹿茸多肽组小鼠玫瑰花环成环率分别高于对照组（生理盐水）33．3％，34．2％，39．6％，差异显著（P〈0．05）。注射8mg／kg鹿茸多肽的小鼠血清溶血素OD值与对照组差异极显著（P〈0．01）。实验组小鼠腹腔巨噬细胞吞噬百分率分别比对照组高22．1％，25．8％，26．5％，差异显著或极显著（P〈0．05或P〈0．01），说明鹿茸多肽具有免疫调节作用，且呈剂量依赖性。注射4，8mg／kg鹿茸多肽组老年小鼠血清超氧化物歧化酶（SOD）活性分别升高51．1％，59．2％，血清丙二醛（MDA）含量分别下降14．9％，16．7％，与对照组差异显著（P〈0．05），说明鹿茸多肽对老年小鼠有较强的抗氧化作用。小鼠负重游泳实验中，注射小鼠存活时间比对照组延长了21．67min（P〈0．05），说明鹿茸多肽具有抗疲劳作用。The velvet antler polypeptides were extracted with acid and alcohol, which showed some bands below 17kDa in SDS-PAGE and a single band in Westem-boltting with the anti-human IGF-1 as the first antibody. Quantitative analysis showed that the polypeptides was 1 - 2 mg/g of green weight by Bradford technique, IGF-1 was 300-400 pg/g by radioimmunoassay （RIA）. Mice were injected with polypeptides 1,4,8 mg/kg of body weight, after six days, the experiments on growth promotion,immunological regulation, anti-lipid peroxidation, anti-fatigue were done. The results showed that： The mean accumulative weight gained in polypeptide group was higher than that of the control group, but not significantly（ P 〉 0.05） ; The rosettes looping rates of the 1,4,8 mg/kg polypeptide groups were 33.3% ,34.2% ,39.6% higher than the control（injected with NS） （ P 〈 0.05） ; OD value of haemolysin in serum of 8 mg/kg polypeptide group was significantly different from the control（ P 〈 0.01） ; The phagocytosis percentage of the 1,4,8 mg/kg polypeptide groups were 22.1%, 25.8 %, 26.5 % higher than the control（ P 〈 0.05, P 〈 0.01）, which indicated that the polypeptide has the function of immunological regulation. The superoxide dismutase （SOD） activities and malondialdehyde （MDA） content in serum of old mice injected with 4,8 mg/kg polypeptide groups 51.1% ,59.2% higher and 14.9%, 16.7% lower respectively than the control group（ P 〈 0.05）, which indicated the anti-lipid peroxidation function of the polypeptides. In the weight swimming experiment, the swimming time of 8 mg/kg polypeptide group was 21.67 mins longer than the control （P 〈 0.05）, which indicated the anti-fatigue function of polypeptides.

关 键 词：鹿茸多肽 生物学活性 免疫调节 抗氧化 超氧化物歧化酶 丙二醛 

分 类 号：TQ936.16[化学工程]