小鼠4、8-细胞胚胎单卵裂球体外培养体系的研究  

Study on in Vitro Cultural System of Blastomeres Isolated from Mouse 4, 8-cell Embryos

作　　者：胡辛怡[1] 周光斌[1] 杨中强[1] 刘国世[1] 朱士恩[1] 

机构地区：[1]中国农业大学动物科技学院,北京100094

出　　处：《中国畜牧杂志》2006年第11期12-15,共4页Chinese Journal of Animal Science

基　　金：农业生物技术国家重点实验室开放课题资助

摘　　要：以CZB为基础培养液,培养小鼠4、8-细胞胚胎单卵裂球,研究葡萄糖、牛磺酸和猪输卵管上皮细胞共培养在其体外发育中的作用。结果表明:牛磺酸添加与否对4、8-细胞胚胎单卵裂球的囊胚发育率分别为29%、30%和14%、14%,无显著性差异(P>0.05)。添加葡萄糖后,4、8-细胞胚胎单卵裂球的囊胚发育率分别为36%和19%,有显著提高(P<0.05)。含有葡萄糖而牛磺酸的添加与否对4、8-细胞胚胎单卵裂球的囊胚发育率分别为36%、38%和19%、20%,无显著性差异(P>0.05)。各组内4、8-细胞胚胎单卵裂球形成的囊胚细胞数分别为(10.44±1.24～(12.43±1.18)和(7.57±0.97)～(8.48±1.16),均无显著性差异(P>0.05)。4、8-细胞胚胎单卵裂球与猪输卵管上皮细胞共培养,其囊胚发育率分别为47%和26%,囊胚细胞数分别为(17.57±1.13)和(11.43±0.92),均高于单一培养(P<0.05)。CZB medium was used as a base medium to study the function of taurine, glucose and porcine oviductal epithelial cells system in the culture of blastomeres isolated from mouse 4,8-cell embryos. These results showed addition oftaurine in CZB did not significantly affect the blastocyst rate of blastomeres isolated from mouse 4, 8-cell embryos （29% vs 30% and 14% vs 14% ） （P 〉 0.05）. Addition of glucose in CZB had a significant effect on the blastocyst rate of blastomeres isolated from mouse 4,8-cell embryos （29% vs 36% and 14% vs 19%） （P〈 0.05）. Addition of taurine did not have a significant effect on the blastocyst rate of blastomeres isolated from mouse 4,8-cell embryos respectively （36% vs 38% and 19% vs 20%） when glucose had been added in CZB （P 〉 0.05）. The cell number of blastocysts derived from blastomeres isolated from mouse 4, 8-cell embryos were not different significantly （10.44 ± 1.24） - （12.43 ±1.18） and （7.57 ±0.97） - （8.48 ±1.16） respectively （P 〉 0.05）. The blastocyst rate of co-cultured blastomeres isolated from mouse 4, 8-cell embryos were 47% and 26%, respectively; the cell number of blastocysts derived from co-cultured blastomeres isolated from mouse 4, 8-cell embryos were 17.57 ±1.13 and 11.43 ±0.92, repectively. These were more than those of blastomeres in the controls（P 〈 0.05）.

关 键 词：畜牧 兽医科学基础学科 卵裂球 体外培养 猪输卵管上皮细胞 小鼠 

分 类 号：S865.130.3[农业科学—野生动物驯养]