光合细菌培养基组成对类胡萝卜素产量的影响  

Effects of Components of Medium for Photo-synthetic Bacteria on its Synthesizing Carotenoid

作　　者：陈德明[1] 韩永斌[1] 顾振新[1] 

机构地区：[1]南京农业大学农业部农畜产品加工与质量控制重点开放试验室,南京210095

出　　处：《微生物学通报》2006年第3期11-17,共7页Microbiology China

基　　金：江苏省高新技术项目(No.BG2004323)

摘　　要：采用响应面法对光合细菌培养基主要成分进行了优化,研究了培养基组成对类胡萝卜素产量的影响。经过逐步回归分析建立了类胡萝卜素产量对培养基主要成分的二次回归模型,其回归方程的决定系数达到了0．958。得到的最适培养基主要组成为:0．81％柠檬酸、0．35%NH4Cl和0．18％玉米浆,类胡萝卜素产量最大预测值达到13．34mg／L,是优化前的2．04倍。The medium of photo-synthetic bacteria synthesizing carotenoid was optimized with response surface methodology. The regression equation expressing the relationship between carotenoid yield and main components of culture medium was established by stepwize analysis. The R-squared in the model of regression equation was 0. 958, which meant the established equation couM predict the carotenoid yield well at the range of factors in this design. It was indicated that the optimum medium components were： 0. 81% citric acid, 0. 35% NH4Cl and 0. 18% corn syrup. On those conditions, it was predicted that the highest production of carotenoid was 13.34 mg/L, which was 2.04 times higher than initial carotenoid yield before optimization.

关 键 词：光合细菌 培养基 类胡萝卜素 响应面法 

分 类 号：Q935[生物学—微生物学]