大鼠脊髓半横断损伤后凋亡相关基因的表达变化  

The changes in the expression of apoptotic correlative genes(Bax,Bcl-2,Fas) in neurons following hemisection spinal cord injury

作　　者：罗伟[1] 赵匡彦[2] 王廷华[2] 

机构地区：[1]解放军总医院神经外科,北京100853 [2]昆明医学院神经科学研究所,云南昆明650031

出　　处：《中华神经外科疾病研究杂志》2006年第3期248-250,共3页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的 探讨脊髓半横断损伤后凋亡相关基因Bcl-2、Bax、Fas在蛋白水平的表达变化规律及神经细胞凋亡的分子生物学机制.方法 在成年SD大鼠脊髓T9～T10间半横断,取损伤位点尾侧段T10节段制作冰冻切片,运用Bcl-2、Bax、Fas兔抗血清以免疫组化亲合素-生物素-过氧化物酶复合物法（ABC）法染色.观察并计数腹角Bcl-2、Bax、Fas的阳性神经元数.结果 Bcl-2、Bax、Fas主要分布于正常大鼠脊髓腹角神经元细胞浆,损伤后腹角Bcl-2、Bax、Fas阳性神经元数在3 d组（n=6）、7 d组（n=6）、21 d组（n=6）均较假手术组（n=6）明显增加（P《0.01）, Bax、Fas阳性神经元数在术后3 d时达高峰,随伤后时间的延长进行性减少（P《0.05）.Bcl-2阳性神经元数在术后7 d时达高峰,3 d组与21 d组比较没有显著差异.结论 脊髓半横断损伤（hSCI）后,由Fas抗原参与的死亡受体途径及Bcl-2、Bax参与的线粒体途径均参与了hSCI后细胞的凋亡过程.Objective To explore the changes in the expression of apoptotic relative genes （Bax, Bcl-2, Fas） in the spinal cord ventral horn neurons in early stage after hemisection spinal cord injury （hSCI）. Methods Hemisection spinal cord injury was performed between T9 and T10 in adult Sprague-Dawtey （SD） rats. The rats were sacrificed 3 d, 7 d and 21 d after operation respectively, and the T10 spinal segments were removed out. Immunohistochemical ABC staining was used and the positive neurons numbers were counted.Results Bax, Bcl-2, Fas positive staining were mainly distributed in cytoplasm of neurons located in the spinal ventral hom in the normal group. The number of Bax, Bcl-2, Fas positive neurons apparently increased in the 3 d （n =6）,7 d （n=6） and 21 d （n =6） groups compared to that of the sham-operational group （P〈0.01）. The Bax, Fas positive ncurons reached a peak at 3 d after injury, and decreased progressively with time following hSCI （P〈0.05）. Bcl-2 positive ncurons reached a peak at 7 d after injury （P〈0.01） with no difference in the number of Bcl-2 positive neurons between 3 d and 21 d groups. There exists high expression of apoptotic relative genes Bax, Bcl-2, and Fas after hSCI. Conclusion A delayed progressive destructive process causes even more severe spinal cord damage in a way of apoptosis. Bax, Bcl-2, and Fas protein are possible critical factors in this process.

关 键 词：脊髓 半横断损伤 BAX Bcl-2 FAS 

分 类 号：R651.3[医药卫生—外科学]