胡麻油烟冷凝物致人胚肺二倍体细胞恶性转化的实验研究  

Malignant Transformation Induced by Siritch Oil Fume Condensates on Human Embryo Lung Diploid Cells

作　　者：李丽萍[1] 刘秀芳[1] 

机构地区：[1]宁夏医学院公共卫生学院环境卫生毒理学教研室,银川750004

出　　处：《宁夏医学院学报》2006年第3期187-189,F0002,共4页Journal of Ningxia Medical College

基　　金：宁夏自然科学基金资助项目(编号:C107)

摘　　要：目的研究胡麻油烟冷凝物致人胚胎二倍体细胞恶性转化作用.方法以人胚肺二倍体细胞株为靶细胞,建立体外转化系统,以胡麻油烟冷凝物作为受试物染毒人胚肺二倍体细胞,观察细胞转化情况,并对转化细胞进行生物学性状检测和鉴定.结果实验剂量范围内的胡麻油烟冷凝物（25～400μg/mL）能够诱导人胚肺二倍体细胞发生失去接触抑制性、饱和密度增大、血清需求降低,ConA凝集能力增强、锚着依赖性丧失等多种与肿瘤形成相关的表型改变,并且剂量-反应关系明显（r=0.972,P＜0.01）.结论胡麻油烟冷凝物具有诱导人胚肺二倍体细胞转化的作用.Objective To study the potential carcinogenic risk of siritch oil fume （SOFC） for human embryo lung diploid cells. Methods Human embryo lung diploid cells were subjected to establish transformation system in vitro. Different concentrations of SOFC were added into the DMEM cultures and co-incubated with human embryo lung diploid cells. The malignant degree of transformation was observed by assessing the biological characteristics of the cells. Results The concentrations of SOFC （25 - 400 μg/mL） could induce the malignant transformation of the human embryo lung diploid cell, such as loss of contact-dependent inhibition, saturated density augmenting, growth at low serum concentration, agglutination by different concentration of ConA, loss of anchorage dependence, etc. Those characteristics associated with malignant transformation and with an obvious dose-response relationship. Conclusion SOFC could induce human embryo lung diploid cell malignant transformation.

关 键 词：胡麻油烟冷凝物 人胚肺二倍体细胞 恶性转化 

分 类 号：X51[环境科学与工程—环境工程]