银诱导大鼠肝脏金属硫蛋白的提纯与鉴定  

Purification and Characterization of Silver-Induced Metallothionein in Rat Liver

作　　者：黄仲贤[1] 郑起[1] 顾伟强[1] 王韵华[1] 

机构地区：[1]复旦大学化学系

出　　处：《高等学校化学学报》1996年第9期1349-1352,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：Ｗｉｓｔａｒ公鼠经腹腔注射ＡｇＮＯ３后可诱导肝脏合成ＭＴ。经匀浆、乙醇沉降、Ｓｅｐｈａｄｅｘ－７５、ＤＥＡＥ－５２两次柱层析，可得到两个亚型。原子吸收测定结果表明：该蛋白分别含７份Ａｇ、２份Ｚｎ和２份Ｃｕ，具有与Ｃｄ５Ｚｎ２－ＭＴ并不相同的二级、三级结构。进一步研究表明，蛋白中伴随Ｃｕ和Ｚｎ的含量与所用诱发剂的种类、数量均有关，且Ｃｕ和Ｚｎ（通过ＭＴ）具有某种微妙的联系。The rat liver metallothionein was induced by subactaneous injections of silver nitrate and was purified to homogeneity by using homogenization, ethanol precipitation, Sephadex G-75 chromatography and chromatography on DEAE-52. Anion exchange chromatography yielded two isoforms of metallothionein, MT-1 and MT-2, in which the molar ratios of Ag+, Zn+ and Cu+ bound are 7,2 and 2,respectively.It was also found that the ratios were slightly affected by the dose of silver nitrate, which indicate that the two endogenous essential metals, Cu and Zn, accompanying silver showed competition with the exogenous heavy metal ions. MT has different selectivities towards metals.

关 键 词：银 金属硫蛋白 金属调节 结合蛋白 层析 大鼠 

分 类 号：Q513[生物学—生物化学] O658.1[理学—分析化学]