番红花红T与表面活性剂的作用及其在标记DNA中的应用  

The Interaction of Safranine T with Anionioc Surfactant and Its Application in Labelling DNA

作　　者：郭祥群[1] 李芳[1] 赵一兵[1] 王冬媛[1] 许金钩[1] 

机构地区：[1]厦门大学化学系材料和生命过程分析科学开放研究实验室

出　　处：《高等学校化学学报》1996年第9期1361-1366,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：对阳离子染料番红花红Ｔ（ＳＴ）在阴离子表面活性剂存在时的溶液状态的吸收光谱和荧光光谱进行了研究．结果表明，低浓度阴离子表面活性剂与ＳＴ形成缔合物，导致ＳＴ的吸收与荧光强度降低；增大表面活性剂的浓度，其分子胶束前预聚集促使染料形成非荧光二聚体，导致荧光急剧猝灭，吸收光谱出现新的特征吸收峰；当表面活性剂浓度大于临界胶束浓度（ＣＭＣ）时，染料二聚体离解，ＳＴ单体增溶于胶团中形成新的高量子产率荧光体，本文还对染料分子在表面活性剂分子胶束前预聚集过程中形成的非荧光二聚体用作脱氧核糖核酸（ＤＮＡ）的荧光探针的可行性进行了初步的研究。The study of the properties of the visible spectra and fluorescent spectra of safranine T(ST)in the solution of anionic surfactants(AS)was reported. The results showed that ST formed complex with AS molecule when the concentration of AS was low, and the absorbance and fluorescence intensities of ST, therefore, decreased.A non-fluorescent dimeric dye was formed on the formation of premiceller aggregation of AS when the concentration of AS was made higher, and thus the fluorescence intensity decresed dramatically,and a new peak in visible spectra appeared.When the surfactant concentration was increased above the critical micelle concentration(CMC), the dimeric dye dissociated, and a new fluophore with a high quantum yield was formed. The posibility of using the non-fluorescent dimeric dye as a fluorescent probe for deoxyribonucleic acid was also studied. The dimeric dye showed a highly DNA binding constant and significant change of fluorescent behaviour was observed when the dimeric dye interacted with double strands DNA. It was obvious that the dimeric dye may be used as a good DNA probe.

关 键 词：番红花红T 表面活性剂 DNA 荧光染料 

分 类 号：TQ617.3[化学工程—精细化工] Q523[生物学—生物化学]