LC-MS法测定比格犬血浆中长春氟宁浓度及其药代动力学  

Determination of vinflunine in Beagle dog′s plasma by LC-MS and study of its pharmacokinetics

作　　者：赵小平[1] 王永升[1] 钟皎[1] 陈燕 柳晓泉[1] 王广基[1] 

机构地区：[1]中国药科大学药物代谢与动力学研究中心,南京210009

出　　处：《中国药科大学学报》2006年第3期242-245,共4页Journal of China Pharmaceutical University

基　　金：国家高技术研究发展计划("八六三"计划)资助项目(No.2003AA2Z347A);江苏省药物代谢动力学重点实验室资助项目(No.BM2001201)~~

摘　　要：目的：建立测定比格犬血浆中长春氟宁浓度的LC-MS方法，用于研究其在犬体内的药代动力学。方法：血浆样品中加入内标非那雄胺，0．25mol／L NaOH碱化后用乙酸乙酯提取，取上清真空挥干，流动相150μL溶解，取40μL进行LC-MS测定。色谱柱为Shimadzu VP-ODS柱（150mm×4．6mm ID，5μm）；流动相为0．01mol／L乙酸铵水溶液-甲醇（20：80），流速为1mL／min。ESI选择正离子检测：长春氟宁，m／z：817．3；非那雄胺，m／z：373．2。结果：线性范围为0．025-12．5μg／mL。日内和日间的精密度均小于7．8％，方法回收率在97．0％-100．2％之间，提取回收率大于80％。结论：该方法选择性好，灵敏度高，操作简便，符合生物样品的测定要求，可应用于长春氟宁在犬体内的药代动力学研究。Aim： To develop a method for the quantification of vinflunine in Beagle dog＇ s plasma by LC-MS, and to examine phannacokinetic characteristics of vinflunine in Beagle dogs. Methods：After being spiked with finasteride as internal standard and alkalization by 0.25 mol/L NaOH, plasma was extracted by ethyl acetate. The organic layer was evaporated and reconstituted with mobile phase. Analysis of plasma was carried out on a Shimadzu VP-ODS column （150 mm ×4.6 mm ID, packed with 5 μm C18 silica RP particle）. The mobile phase was consisted of methanol-10 mmoL/L ammonium acetate buffer （80：20） with the flow rate of 1.0 mL/min. LC-MS was performed in the selected ion monitoring （SIM） mode with target ions at m/z：817.3 for vinflunine and m/z：373.2 for finasteride. Results： Calibration plots for vinflunine related to internal standard, finasteride, were linear over the concentration range of 0.025 - 12.5 μg/mL. The variability values of intra-assay and inter-assay were both less than 7.8%. The relative recovery of vinflunine from plasma was in the range of 97.0% - 100.2%. Conclusion： The established LC-MS method is simple, accurate, sensitive and applicable for pharmacokinetie study of vinflunine in dogs.

关 键 词：长春氟宁 LC-MS 比格犬 药代动力学 

分 类 号：R96[医药卫生—药理学]