西维因和蝇毒磷的同步荧光光谱解析及应用研究  

Resolution of Synchronous Fluorimetric Spectrum for Carbaryl and Coumaphos and Its Application Study

作　　者：张国文[1] 潘军辉[2] 倪永年[3] 

机构地区：[1]南昌大学食品科学教育部重点实验室,江西南昌330047 [2]南昌大学食品科学与工程系,江西南昌330047 [3]南昌大学化学系,江西南昌330047

出　　处：《光谱学与光谱分析》2006年第6期1092-1095,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20365002);江西省教育厅(赣教技字[2005]-71号)资助项目

摘　　要：研究了西维因、蝇毒磷的荧光行为，发现在pH3．0的Britton-Robinson缓冲介质中，两种农药均能产生较强的的荧光，但光谱相互重叠，当以波长差△λ=60nm进行同步荧光扫描，它们的同步荧光光谱得到较好的分离，同步荧光峰（以激发波长表示）分别位于280和322nm，可同时分别对其进行定量测定。西维因和蝇毒磷的线性范围分别为0．016-0．384μg·mL^-1和0．016～0．320μg·mL^-1；检出限分别为0．015和0．010μg·mL^-1。混合样本分析无需分离，方法简单、快速，用于蔬菜、水果、大米和水样等测定，结果满意。The synchronous fluorimetric method for the simultaneous determination of carbaryl and coumaphos is described. In the Britton-Robinson buffer at pH 3.0, synchronous scanning with △λ= 60 nm was carried out, and the overlapped fluorimetric spectra were better separated. The two synchronous fluorimetric peaks are located at 280 and 322 nm respectively, and can be used for the determination of these two pesticides. No preliminary separation is needed. The method is simple, rapid and successfully applied to the analysis of food samples. The linear ranges of the calibration curves for carbaryl and coumaphos are 0. 016-0. 384μg·mL^-1 and 0. 016-0. 320μg·mL^-1 , respectively. The limits of detection are 0. 015μg·mL^-1 for carbaryl, and 0. 010μg·mL^-1 for eoumaphos.

关 键 词：同步荧光光谱法 西维因 蝇毒磷 农药 

分 类 号：O657.3[理学—分析化学]