蝴蝶兰花发育相关基因pPI9的克隆与表达分析  

Cloning and Expression Analysis of a Phalaenopsis Flowering-related gene pPI9

作　　者：郭滨[1] 陈东红[1] 戴薇[1] 魏星[1] 明凤[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室生物多样性科学研究所生物多样性与生态工程教育部重点实验室,上海200433

出　　处：《复旦学报（自然科学版）》2006年第3期277-282,共6页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金资助项目(30300193)

摘　　要：为研究具有复杂而特异花型的蝴蝶兰花发育的分子机制,利用RT-PCR方法从蝴蝶兰花瓣总RNA中分离出834 bp长的cDNA片断.序列分析表明,该cDNA片断与拟南芥的PI基因有60%的同源性,命名为pPI9.它包含一个开放阅读框,具有编码24.5 ku蛋白质的能力.推导的氨基酸序列中,N端包含一个完整的MADS盒,C端有明显的PI基序.半定量PCR结果表明该基因只在植株的生殖器官中表达,而在营养器官中不表达,推测其可能参与花形态建成过程的调节.To investigate the molecular mechanism of floral development of the complex and special Phalaenopsis flower,an 834 bp fragment was isoted from the total RNA of Phalaenopsis petals by RT-PCR. It shows 58% amino acid sequence identity with PISTILIATA from Ambidopsis, and was named pPI9. The deduced amino acid sequence has a complete MAilbox domain in the N-terminal and a PI-rnotff in the C-terminal. The results of semi-quantitative PCR showed that it only expressed in the reproductive organs, which suggested that it was probably involved in the regulaticn of floral morphogenesis.

关 键 词：蝴蝶兰 MADS-BOX基因 花形态建成 RT-PCR 

分 类 号：Q946[生物学—植物学]