多肽Tat与CdTe量子点共价偶联后的跨膜转运  

Intracellular Delivery of CdTe Quantum Dots Conjugated with Polypeptide Tat

作　　者：薛凤丽[1] 卢大儒[2] 郭佳[3] 马炯[4] 苏勇[1] 

机构地区：[1]华东理工大学生物工程学院,上海200237 [2]复旦大学遗传学研究所 [3]复旦大学高分子科学系,上海200433 [4]复旦大学物理系,上海200433

出　　处：《复旦学报（自然科学版）》2006年第3期298-302,共5页Journal of Fudan University：Natural Science

摘　　要：利用偶联剂EDC将具有跨膜转导功能的多肽Tat与水溶性CdTe量子点进行偶联,并用超滤膜反复超滤纯化,然后比较偶联产物和游离量子点进入PLC细胞的量.对接有多肽的量子点进行研究,结果表明,其荧光发射光谱发生了红移.与游离的量子点相比,修饰后的量子点具有更高的细胞膜穿透性,更容易穿过细胞膜进入PLC细胞内部.The CdTe QDs were covalently conjugated with polypeptide Tat by linker EDC. The Tat can deliver exogenous molecules into living cells. The conjugation was purified by ultrafiltration film. A red shift of luminescence of Tat-QDs was observed eomparing with that of unmodified QDs. The intracellular delivery of the Tat conjugated CdTe QDs （Tat-QDs） to living PLC cells was eompared with that of the CdTe QDs, which indicated that the Tat-QDs can enhance their intracellular delivery.

关 键 词：量子点 TAT 碳二亚胺法 

分 类 号：Q334[生物学—遗传学]