纳洛酮对脑震荡大鼠学习记忆和海马CA3区锥体细胞内钙离子的影响  

Effects of naloxone on spatial learning and memory deficits following concussive brain injury in rats together with concentration of calcium in hippocampus CA3 pyramidal cells

作　　者：段新 郭昌茂[2] 马光瑜[2] 

机构地区：[1]顺德区伍仲珮纪念医院老年科,广东佛山528333 [2]广东省食品药品监督管理局,广东广州510080

出　　处：《中国新药与临床杂志》2006年第6期404-407,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：广东省医学科学基金(A2001459)

摘　　要：目的：研究纳洛酮对大鼠脑震荡后空间学习记忆能力的影响及机制。方法：用金属单摆打击装置制作SD大鼠脑震荡模型，随机分为3组：假打击组，脑震荡组，脑震荡＋纳洛酮组：用Morris水迷宫训练方法，评价动物的空间学习记忆能力，用激光共聚焦显微镜观察海马CA3区锥烙细胞内钙离子荧光量值。结果：打击后d8～13脑震荡组隐匿平台逃避潜伏期明显比假打击组长（P〈0．01），穿越原平台位置水域的次数明显比假打击组减少（P〈0.01），海马CA3区锥体细胞内游离钙荧光量平均值（3．0±s0．6）×10^6较假打击组（1．0±0．3）×10^6明显增高（P〈0．01）：脑震荡＋纳洛酮组隐匿平台逃避潜伏期明显比脑震荡组缩短（P〈0．01），穿越原平台位置水域的次数明显比脑震荡组多（P〈0．01），海马CA3区锥体细胞内游离钙荧光量平均值（0．9±0．4）×10 ^6较脑震荡组明显：成低（P〈0．01）：结论：纳洛酮能显著减轻大鼠脑震荡后的空间学习记忆障碍，其机制可能与其防止海马神经细胞内钙离子增高有关．AIM： To investigate the effects of naloxone, a competitive opioid receptor antagonist, on spatial learning and memory deficits following concussive brain injury in rats and its possible neural mechanism. METHODS：A metallic single pendulum-striker concussive device was used to duplicate the animal model of concussive brain injury in SD rats. Rats were randomly divided into three groups： sham injury group, brain concussion group, and naloxone treatment group. Spatial learning and memory abilities were evaluated with a series of training tasks to find a hidden platform in Morris Water Maze on d 8-13 post-concussion. Laser scanning confocal microscope and Fluo-3 were used to measure intracellular calcium fluorescence pixel values of the hippocampus CA3 pyramidal cells. RESULTS： The escape latencies in brain concussion group were statistically longer than those in sham injury group（P 〈 0.01 ）. The number of crossing the former site of the removed hidden platform in brain concussion group were statistically less than that in sham injury group （P 〈 0.01）, and the fluorescence pixel value of intracellular calcium of hippocampus CA3 pyramidal cells in brain concussion group（ （3.0 ±s 0.6） ×10^6）was significantly increased compared with sham injury group （（1.0 ± 0.3）×10^6） （P 〈 0.01 ）. Meanwhile, the escape latencies in naloxone treatment group were statistically shorter than those in brain concussion group （P 〈 0.01 ）, the number of crossing the former site of the removed hidden platform in naloxone treatment group was statistically more than that in brain concussion group （P 〈 0.01 ）, and the fluorescence pixel value of intracellular calcium of hippocampus CA3 pyramidal cells in naloxone treatment group （（0.9 ± 0.4）×10^6）was significantly decreased than that of the brain concussion group. CONCLUSION： Spatial learning and memory deficits were observed following concussive brain injruy in rats,naloxone can attenuate this deficits. Its mechanism may be con

关 键 词：纳洛酮 大鼠 脑震荡 钙 离子 空间学习记亿 

分 类 号：R979.3[医药卫生—药品] R965[医药卫生—药学]