蛇毒α-神经毒素对海洛因成瘾复吸大鼠脑神经元超微结构的影响  

THE EFFECT OF α-NEURONAL DOTOXIN OF SNAKE VENOM ON THE NEURONAL ULTRASTRUCTURES IN THE BRAINS OF THE HEROIN READDICTED RATS

作　　者：叶峻[1] 韦献良[1] 韦世秀[2] 蒙子卿[3] 刘佳娟[4] 廖章鼎[1] 

机构地区：[1]广西医科大学解剖学教研室,南宁530021 [2]广西医科大学医学实验中心 [3]广西医科大学附属肿瘤医院药理室 [4]广西医学实验动物中心

出　　处：《广西医科大学学报》2006年第1期12-14,共3页Journal of Guangxi Medical University

基　　金：广西壮族自治区科学研究与技术开发项目(桂科攻0015050);广西教育厅科研项目(2003年)

摘　　要：目的:探讨蛇毒α-神经毒素对海洛因复吸大鼠脑神经元超微结构的影响。方法:建立海洛因成瘾、脱毒,复成瘾、脱毒,再成瘾、脱毒3个阶段大白鼠模型,动物设阳性对照组、美沙酮-丁丙诺啡-可乐定联合治疗组,高、低剂量蛇毒-神经毒素组共4个组。电镜下观察各组大鼠脑神经元超微结构变化。结果:海洛因复吸大鼠脑神经元出现变性、凋亡、胀亡等超微结构变化,阳性对照组随着复吸次数增加,脑神经元病变百分比依次增加;高剂量蛇毒治疗组脑神经元超微变化与美沙酮-丁丙诺啡-可乐定治疗组相近;低剂量蛇毒治疗组脑神经元超微变化与阳性对照组相近。结论:海洛因复吸大鼠脑神经元出现广泛性超微病理结构改变,随复吸次数增多而病变加重;高剂量蛇毒α-神经毒素能减轻海洛因复吸大鼠脑病理损害。Objective：To investigate the effect of a-neuronal dotoxin of snake venom on the neuronal ultrastructures in the brains of the heroin re-addicted rats. Methods：We set up heroin addiction and re-addiction models with the processes of heroin addiction-abstaining treatment-heroin re-addiction-re-abstaining treatment -heroin addiction for the third time-abstaining theatment for the third time. Rats were divided randomly into 4 groups： the control group, Methadone-Buprenorphine-clonidine group, high and low dose of α-neuronal dotoxin of snake Venom group. The changes of the neuronal ultrastructures in the brains of the rats from all groups were observed under electronic microscope. Result：Degeneration, apoptosis and oncosis were found in the neuron of the heroin re-addicted rats＇ brains. The changes of neuronal ultrastructures of control group increased with the increase of re-addicting times. The changes of the neuronal ultrastrnctures in the brains of the high dose of α-neuronal dotoxin of snake Venom group were similar to the those of Methadone-Buprenorphine-Clonidine group. The changes of the neuronal ultrastructures in the brains of the low dose of α-neuronal dotoxin of snalke venom were similar to those of the control gronp. Conclusion： widespread lesions have been found and were more severe in the re-addicting times. High dose of α-neuronal dotoxin of snake venom could attenuate the neurn lesions.

关 键 词：海洛因 成瘾 复吸 神经元 超微结构 蛇毒 

分 类 号：R965[医药卫生—药理学]