眼镜王蛇毒L-氨基酸氧化酶诱导NACC细胞凋亡作用的研究  

STUDY OF APOPTOSIS OF HUMAN PALATAL SALIVARY GLAND OF ADENOID CYSTIC CARCINOMA INDUCED BY L-AMINO ACID OXIDASE OF KING COBRA

作　　者：黄红坤[1] 黎肇炎[1] 李佳荃[2] 黄爱民[1] 

机构地区：[1]广西医科大学蛇毒研究所,南宁530021 [2]广西医科大学医学科学实验中心

出　　处：《广西医科大学学报》2006年第1期22-25,共4页Journal of Guangxi Medical University

摘　　要：目的:研究眼镜王蛇毒L-氨基酸氧化酶(LAAO)诱导人类腭部小涎腺腺样囊性癌细胞系(NACC)细胞凋亡的作用。方法:采用倒置相差显微镜、H·E染色及透射电镜定性的方法,观察LAAO引起NACC细胞凋亡形态学改变,采用TUNEL半定量计算凋亡指数(AI)方法观察NACC细胞经不同浓度LAAO处理后引起细胞凋亡的情况。结果:倒置相差显微镜及H·E染色光镜观察、透射电镜观察可见典型细胞凋亡形态学改变,并可见凋亡小体形成。TUNEL半定量计算AI值,LAAO处理组与对照组比较差异有统计学意义(P<0·01),且AI值高低与LAAO浓度高低有关。结论:眼镜王蛇毒LAAO能诱导NACC细胞发生凋亡。Objective：To study the effect of L-amino acid oxidase （LAAO） of king cobra venom-induced apoptosis of human palatal Salivary gland of adenoid cystic carcinoma（NACC）. Methods： Light microscope, H · E stain and transmission electronic microscope were used to detect the morphological changes of the apoptosis of NACC cell line and to detect the apoptosis index（AI） of NACC cell line in different concentration of LAAO by using TUNEL method. Result： LAAO of king cobra venom could induce NACC cell apoptosis demonstrated by the typical morphological changes, and apoptotic bodies was observed under in transmission electronic microscope. AI of NACC cells was significantly different between LAAO groups and control groups （ P〈0.01）, and AI was higher in the high concentration group. Conclusion： LAAO of king cobra venom can induce the apoptosis of NACC cells.

关 键 词：小涎腺腺样囊性癌 蛇毒 L-氨基酸氧化酶 细胞凋亡 

分 类 号：R996.3[医药卫生—毒理学]