预处理对兔缺血再灌注损伤心肌的保护作用  

Protective Effect of Preconditioning on Myocardial Ischemia-Reperfusion Injury in Rabbit

作　　者：代友平[1] 唐国华[1] 习水平[1] 钟志生[1] 

机构地区：[1]咸宁医学院内科,江苏省南京医科大学第二附属医院心内科

出　　处：《中国循环杂志》1996年第5期308-310,共3页Chinese Circulation Journal

摘　　要：目的：确定顶处理对兔缺血再灌注损伤心肌是否具有保护作用和氧自由基在兔预处理机制中的作用。方法：采用麻醉开胸兔急性心肌缺血再灌注损伤模型。心肌梗死面积和危险区分别采用组织学和荧光粒子技术测定。实验分４组：对照组（ｎ＝９），预处理组（ｎ＝８），高剂量超氧化物歧化酶预处理组（ＨＤＳＯＤ－ＰＣ，ｎ＝８）和低剂量超氧化物歧化酶预处理组（ＬＤ－ＳＯＤ－ＰＣ，ｎ＝９）。左冠状动脉回旋支４次５分钟缺血和５分钟再灌注完成预处理。对照用于３０分钟缺血后再灌注１２０分钟；其余各组在预处理后的缺血与再灌注同对照组。结果：梗死面积占危险区百分比在对照组与预处理组、ＨＤ－ＳＯＤ－ＰＣ组和ＬＤ－ＳＯＤ－ＰＣ组之间差异有统计学显著意义（Ｐ均＜０．０５）；预处理组、ＨＤ－ＳＯＤ－ＰＣ组和ＬＤ－ＳＯＤ－ＰＣ组之间差异无统计学显著意义。结论：预处理对兔缺血再灌注损伤心肌有保护作用，氧自由基在该预处理机制中不起明显作用。Objectives:To determine whether preconditioning has protective effect and the effect of oxygen free radicals on myocardial ischemia-reperfusion injury in rabbit preconditioning.Methods:A model of acute ischemia-reperfusion injury was produced in open chest anaesthetized rabbits. Myocardial infarct size and area at risk were determined by histology and fluorescent particles,respectively. Rabbits were divided into four groups: control group(C,n=9), preconditioning group (PC,n=8), high-dose superoxide dismutase (SOD) treated preconditioning group (HD-SOD-PC,n=8,SOD 3×104 U·kg(-1)/60 min iv drip prior to PC) and low-dose SOD-PC (LD-SOD-PC,n=9,SOD 1.5×104U·kg(-1)/60 min iv drip prior to PC)group. Preconditioning (PC)was performed with four episodes of 5 min ischemia (by ligation of the circumflex branch of the left coronary artery)and 5 min reperfusion followed by 30 min ischemia and 120 min reperfusion as in control group.Results: The difference in infarct size as the percent of area at risk was significant between the control group and the PC group, the HD-SOD-PC group,and the LD-SOD-PC group(all P<0.05),but the difference between the latter three groups was not significant.Conclusion: Ischemic preconditioning protects the rabbit myocardium from ischemic injury; and free radicals are unlikely to play a major role in the preconditioning in rabbit.

关 键 词：心肌缺血 再灌注 氧自由基 

分 类 号：R541.405[医药卫生—心血管疾病]