精灵口服液对腺嘌呤致大鼠精核蛋白异常的影响  

Effect of Jingling Orally Liquid on Protamine Abnormity of Big Rats Induced by Adenine

作　　者：丁彩飞[1] 鲍严钟[1] 杨欣[1] 颜志中[1] 李海松[2] 

机构地区：[1]浙江省中西医结合医院生殖医学科,杭州310003 [2]北京中医药大学东直门医院

出　　处：《浙江中西医结合杂志》2006年第7期397-399,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医管理局基金项目(No:2001C094)

摘　　要：目的探讨精灵口服液治疗男性不育症的作用机制和环节。方法Wistar系大鼠40只,分正常组10只,用腺嘌呤灌胃法复制睾丸功能损害的大鼠肾阳虚模型30只,随机分模型组、治疗Ⅰ组、治疗Ⅱ组各10只。治疗Ⅰ、Ⅱ组分别灌胃精灵口服液3.6g/kg、1.8g/kg,观察大鼠睾丸和附睾精子的精核蛋白(RP)及精-组蛋白的构成比的变化。结果精灵口服液可提高大鼠睾丸、附睾内精子精核蛋白和过渡型蛋白1(TP1)的含量,降低总组蛋白与精核蛋白(TH/RP+TP1、TH/RP)的比值,改善大鼠睾丸、附睾内精子细胞的核蛋白构成。结论精灵口服液能促进精核蛋白的生物合成,纠正蛋白-精核蛋白取代反应(HPRR)阻滞,给精子核以适当“包装”,使其在生理状态下具备生殖能力。Objective： To investigate the mechanism of Jingling orally liquid in treating infertile male rats. Methods： Big rats with kidney-Yang deficiency and injured testicular function were made by stomach perfused adenine, and divided randomly into normal group, model group, treatment Ⅰ group, treatment Ⅱ group and Jingling orally liquid group. The variation of testicle, protamine and the constitutive ration of protamine-histone of big rats were observed. Results： Jingling orally liquid could increase the content of testicle, protamine and transition proteins 1 （TP1）, and improve the constitution of protamine in germ cells. Conclusion： Jingling orally liquid can promote the biosynthesis of protamine, correct the block of HRRP, and provide a casting of sperm nucleus, thus get the reproductive ability.

关 键 词：不育大鼠 精灵口服液 精核蛋白 

分 类 号：R285.5[医药卫生—中药学]