RP-HPLC测定血浆中间硝苯地平的浓度  

Determination of M-nifedipine in Beagle Dogs by RP-HPLC

作　　者：杨志福[1] 周四元[2] 杨铁宏[2] 梅其炳[2] 

机构地区：[1]中国人民解放军第四军医大学西京医院药剂科 [2]药学系药理学教研室,陕西西安710032

出　　处：《解放军药学学报》2006年第3期219-222,共4页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的 建立高效液相色谱法测定Beagle犬血浆中间硝苯地平（m-nifedipine，m-Nif）的浓度，并研究其代谢动力学规律。方法 采用正交设计优选色谱和提取条件，血浆样品中m-Nif经碱化、萃取后，用反相高效液相色谱法（RP-HPLE）进行分析测定。色谱分析柱为C18柱（250ram×4．6mm，5μm）；流动相为甲醇-10mmol·Ll^-1磷酸盐缓冲液pH5（83：17）；流速为1．0ml／min；检测波长为350nm；内标为MN9201（二氢吡啶类）。结果线性范围为10-500ng·ml^-1，最低检测浓度为10ng·ml^-1，低、中、高3种不同浓度的方法回收率分别为110．84％、105．60％、104．23％；提取回收率分别为92．75％、104．46％、105．12％，日内RSD分别为9．70％、3．05％和1．75％；日间RSD分别为14．53％、7．21％和5．44％。结论 本方法稳定、简便、准确，可用于Be础犬血浆中m-Nif的浓度测定及药代动力学研究。Aim To develop a sensitive and rapid HPLC method for determination of m-Nifedipine （m-Nif） and to study its pharmacokinetics in Beagle dog.Methods A reverse phase HPLC（RP-HPLC） and extraction methods were selected by the orthogonal experimental design.Mter being alkalinized and extracted, m-Nif and MN9201 were analyzed by RP-HPLC with Cls column（250mm×4.6mm, 5μm）, The mobile phase consisted of methanol-10mmol.L^- 1 phosptat buffe pH5 （83：17） at the flow rate of 1.0ml/min; the UV detection wave-length was 350nm. Results The calibration curve was shown to be linear over the range from 10 to 500ng. ml^- 1 （ r = 0. 999 4） ： The lowest detectable concentration was 10ng. ml^-l.The recoveries method were 110.84% ,105.60% and 104.23% respectively; the extract recoveries were 92. 75 %, 104.46 % and 105.12 % respectively at the concentration of 20,150 and 400ng.ml^-1. The withinday RSD was 9. 70% ,3.05% and 1.75% respectively and the between-day RSD was 14.53% ,7.21% and 5.44% respectively at the concentration of 15,100 and 500ng.ml^-1. Conclusion This simple, sensitive and accurate method can be applied to determining the phannacokinetic of m-Nif in the plasma of Beagle dogs.

关 键 词：间硝苯地平 药物代谢动力学 高效液相色谱 

分 类 号：R969.1[医药卫生—药理学]