纤维蛋白胶外周支持对大鼠颈静脉移植物内膜的影响  

The effect of fibrin glue perivenous on endothelium of rat vein grafts

作　　者：宋民[1] 罗国华[1] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院心血管外科,北京100037

出　　处：《中国现代医药杂志》2006年第6期1-3,共3页Modern Medicine Journal of China

摘　　要：目的探讨纤维蛋白胶可降解血管外周支持对静脉移植物急性期内膜的影响。方法建立大鼠颈总动脉自体颈静脉移植模型,按照有无纤维蛋白胶血管外周支持,分为纤维蛋白胶外周支持组、无外周支持组,每组24只大鼠。移植术后2小时切除移植静脉,进行扫描电镜检查,用Ⅷ因子染色的方法检测内皮的缺失情况。结果Ⅷ因子染色显示,纤维蛋白胶外周支持组的静脉移植物内皮层基本保持完整,而无外周支持组的静脉移植物内皮层破坏严重。扫描电镜检查显示,纤维蛋白胶外周支持组的静脉移植物内膜大致结构正常,无外周支持组的静脉移植物血管内膜结构破坏严重。结论纤维蛋白胶血管外周支持,能够改善静脉移植物的应力状态,减轻动脉循环引起的急性机械性损伤,保持血管内皮层的完整性。Objective To study the effect of fibrin glue perivenous on the acute injury of rat vein grafts. Methods Rat jugular vein grafting models made by transplanting autologous external jugular vein graft into the carotid artery in 24 rats, were divided into FS group （with the fibrin glue supported vein grafts）, NS group （without perivenous supported vein grafts）. The vein grafts were harvested 2 hours after operation. The vein wall segments were taken for histologic, electron microscopical and immunohistochemistrical analysis. Results In NS group, histologic analysis showed： in FS group, vein grafts had almost the intact endothelial layer which was not adhesion of platelet and had a little of lymphocyte; in NS group, endothelial layer of vein grafts had severe damage, was adhision of platelet and had a lot of lymphocyte. Electron microscopical examination in NS group showed an extensive destruction of the basal lamina and loss endothelium, but vein grafts have insignificant damage of endothelial layer in NS group and almost the intact. Light microscopy with Ⅷ as endothelial marker showed severe damage of factor but the endothelium remained intact endothelial layer. Conclusions The fibrin glue perivenous support can improve stress of vein grafts, prevent endothelium of vein grafts from acute injury by arterial circulation and alleviate inflammation of vein grafts.

关 键 词：静脉移植 纤维蛋白胶 血管外周支持 

分 类 号：R654.3[医药卫生—外科学]