藏红花不同类型愈伤组织器官形态发生能力的差异  

Differences on organogenesis of callus types in saffron

作　　者：杨永华[1,2] 郭子彪[1,2] 萧凤 

机构地区：[1]南京大学生物科学与技术系 [2]南京农业大学农业与生命科学学院 [3]云南农业大学农业科学技术学院

出　　处：《植物资源与环境》1996年第3期63-64,共2页Journal of Plant Resources and Environment

基　　金：国家农业部"八五"生物技术项目;江苏省科学技术委员会应用基础研究项目

摘　　要：藏红花不同类型愈伤组织器官形态发生能力的差异杨永华郭子彪（南京大学生物科学与技术系，南京２１００９３）（南京农业大学农业与生命科学学院，南京２１００９５）萧凤（云南农业大学农业科学技术学院，昆明６５０２０１）Ｄｉｆｅｒｅｎｃｅｓｏｎｏｒｇａｎｏｇｅ...The stigma of saffron ( Crocus sativus L.) contains natural pigments of economic importance. The present experiment deals with the differences on organogenesis of saffron calli for efficient and rapid micropropagation. The calli could be classified into four groups: (1) opaque yellow, compact, granular and embryonic callus (A type) proliferating shoots and roots in frequencies of 94% and 89% respectively; (2) dull purple, compact and tumourlike callus (B type) showing high root differentiation frequency at about 90%, but very low shoot one; (3) white, fragile callus of twisted pieces (C type) growing slowly at the shoot and root differentiation frequencies of 8% and 51% respectively; (4) opaque white, fragile and lumpish callus (D type) multiplying fast, losing the morphogenetic capacity, but having potentials to select saffron somaclonal lines of high pigment contents. Among the explants, young leaves and buds were easily induced to form A type calli, about at the high frequencies of 95% in 2～3 weeks; tubers were induced to produce the calli at 80% in 6～7 weeks, implying that the former are suitable sources of the explants to select embryonic calli rapidly.

关 键 词：藏红花 器官形态发生 芽分化 根分化 

分 类 号：S567.239[农业科学—中草药栽培]