绿宝石喜林芋叶片培养及植株再生  

Foliar Culture and Plant Regeneration of Philodendron Erubescens

作　　者：陈秀蕙[1] 潘学峰[1] 刘国民[1] 李任珠[1] 

机构地区：[1]海南大学农学院

出　　处：《热带作物学报》1996年第1期96-101,共6页Chinese Journal of Tropical Crops

摘　　要：结果表明：诱导绿宝石喜林芋叶片产生愈伤组织频率最高的培养基是ＭＳ＋ＢＡ０．０５ｍｇ／Ｌ＋２，４－Ｄｌ．０ｍｇ／Ｌ。最适于愈伤组织分化的基本培养基是Ｎ＿６；３ｍｇ／Ｌ的ＢＡ、ＺＴ和ＫＪ均能诱导愈伤组织产生不定芽，其中以ＢＡ为好；ＣＨ对愈伤组织的分化有促进作用。Ｎ＿６＋ＢＡ７ｍｇ／Ｌ＋ＩＢＡ１．０ｍｇ／Ｌ对不定芽的增殖效果较好，３０ｄ可增殖９．４倍。１／２ＭＳ＋ＩＢＡ０．５ｍｇ／Ｌ诱导生根效果好。试管苗移栽的成活率高达９８％以上。Leaf of Philodendron erubescens was induced for plantlet regeneration on MS,H, N6or KC medium. Experimental results demonstrated that the medium MS+ BA0.5mg/L+2,4-Dl.0mg/L gave the highest frequency of leaf callus inducing and that the optimum medium forcallus differentiation was N6.Leaf calli couldbe induced to produce adventitious buds by all thecytokinins BA,ZT and KT of which BA was best effective. Callus differentiation was encour-aged by CH at 500mg/L. N6 + BA7.0mg/L+IBA1.0mg/L was found good for proliferation ofadventitious buds, the muItiplication rate being 9.5 times in 30 days.1/2 MS + IBA0. 5mg/Lwas favourable for rooting. The transplanted plantlets had a survival rate as high as 98%.

关 键 词：绿宝石喜林芋 愈伤组织 不定芽 植株再生 

分 类 号：S687.303.5[农业科学—观赏园艺]