异丙酚对大鼠海马神经元NMDA受体通道电流的影响  

Effect of propofol on N-methyl-D-aspartate subtype of glutamate receptor in cultured rat hippocampal neurons

作　　者：秦晓辉[1] 杨胜[1] 米卫东[1] 张宏[1] 

机构地区：[1]解放军总医院麻醉科,北京市100853

出　　处：《中华麻醉学杂志》2006年第5期407-410,共4页Chinese Journal of Anesthesiology

摘　　要：目的研究异丙酚对大鼠海马神经元N-甲基-D-天冬氨酸(NMDA)受体通道电流的影响。方法体外培养新生Wistar大鼠海马神经元8-12 d,采用全细胞膜片钳技术和压力喷射给药方式,钳制电压为-80 mV,记录3、48μg／ml异丙酚对100 μmol／L NMDA诱发NMDA受体通道电流的影响;然后用γ-氨基丁酸(GABA)受体拮抗剂荷包牡丹碱100 μmol／L阻断GABAA受体,观察3、48μg／ml 异丙酚对NMDA受体通道电流的影响。结果 3、48 μg／ml异丙酚抑制自发兴奋性突触后电流并直接激动GABAA受体,使Cl-内流,产生外向超极化的GABA电流,从而间接抑制NMDA受体通道电流。用荷包牡丹碱100 μmol／L阻断GABAA受体后,3、48 μg／ml异丙酚仍可抑制100 μmol／L NMDA诱发的 NMDA受体通道电流(P<0．05)。结论异丙酚通过激活GABAA受体影响NMDA受体通道电流,对 NMDA受体通道也有直接抑制作用。Objective To investigate the effect of propofol on N-methyl-D-aspartate （NMDA） receptormediated currents.Methods Hippocampal neurons were prepared from newborn Wistar rats and cultured for 8-12 days. Whole cell currents were recorded using patch-clamp technique and cells were voltaged-elamped at - 80 mV. 100μmol-L^-1 NMDA and 3 or 48μg·ml^-1 propofol were applied with a multi-pipe ejection system. GABAA receptor was then blocked with 100 μmol· L^-1 bieueulline to investigate the effect of propofol on NMDA receptor without the influence of GABAA receptor. Results Run-down of INMDA induced by 100μmol·L^-1 NMDA applied to neuronswhieh were cultured for 8-12 days was 15% ±8%. Propofol 3 or 48μg·ml^-1 significantly inhibited spontaneous excitatory postsynaptie currents and elicited a C1^- -mediated response by direct activation of GABAA receptor in the absence of GABA. therefore produced a reversible inhibition of whole cell currents activated by NMDA. After the GABAA receptor was blocked by 100 μmol·L^-1bieueuline propofol still inhibited NMDA currents slightly. Conclusion Propofol inhibits the NMDA subtype of glutamate receptor mainly through activation of GABAA receptor. It can also directly suppress the NMDA receptor slightly.

关 键 词：二异丙酚 海马 神经元 受体 N-甲基-D-天冬氨酸 

分 类 号：R96[医药卫生—药理学]