脂可平对实验性早期动脉粥样硬化大鼠主动脉ICAM-1表达的影响  

Effects of Zhikeping on the Expression of Aortic ICAM-1 in a Rat Model of Experimental Early Atherosclerosis

作　　者：陈志强[1] 丁奇峰[1] 尹智炜[1] 张秀君[1] 黄怀鹏 李红霞[1] 

机构地区：[1]河北医科大学中西结合研究所,石家庄050017 [2]神威药业有限公司,石家庄051430

出　　处：《天然产物研究与开发》2006年第3期394-397,共4页Natural Product Research and Development

摘　　要：研究脂可平对大鼠早期动脉粥样硬化氧自由基及细胞间粘附分子(ICAM1)表达的影响。应用高脂饲料复制SD大鼠早期动脉粥样硬化模型,分别给以脂可平、辛伐他汀片混悬液灌胃,实验10周后,酶比色法检测各组大鼠血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,用免疫组化和RTPCR方法检测主动脉壁ICAM1及其mRNA的表达水平。两治疗组血清MDA含量均明显降低,SOD活性显著升高,ICAM1及其mRNA的表达水平显著下降,且脂可平优于辛伐他汀。本实验说明脂可平抗动脉粥样硬化作用的机制可能与其抗氧化、抗粘附等保护血管内皮细胞功能密切相关。To investigate the effects of Zhikeping on the expression of oxyradical and intercellular adhesion molecule （ICAM-1） of experimental early atherosclerosis blood vessel. The model of SD rat early atherosclerosis was induced by cholesterol diet;the suspension of Zhikeping and simvastatin were administered intragastrically, respectively. After 10 weeks, the serum superoxide dismutase （SOD） and maleic dialdehyde （MDA） were detected by enzyme colorimetric method.ICAM-1 and its expression of mRNA in aortic wall were detected by immunohistochemistry and RT-PCR,respectively. The results show that the serum superoxide dismutase （SOD） and maleic dialdehyde （MDA） of treated groups were obviously improved compare with those of the model group, ICAM-1 and its expression of mRNA was decreased obviously,and the effect of Zhikeping was better than that of simvastatin. It is suggested the mechanism of anti-atherosclerosis of Zhikeping have close relationship with the function of its anti-oxidizing and anti-adhesiveness that can protect vessel endothelial cell.

关 键 词：脂可平 动脉粥样硬化 氧自由基 细胞间粘附分子 

分 类 号：R285[医药卫生—中药学]