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出 处:《天津农业科学》2006年第2期18-19,共2页Tianjin Agricultural Sciences
摘 要:以花叶玉簪甜心、法兰西、王冠等的芽为外植体,以MS+6-BA5mg/L+NAA0.2mg/L为芽诱导培养基,MS+6-BA2mg/L+NAA0.5mg/L为增殖培养基,可以获得较好的增殖效果。在1/2MS+NAA0.1mg/L+0.2%活性炭的培养基上生根效果好,用腐叶土和珍珠岩4∶1比例的基质移栽苗时其成活率可达95%以上。Tissue culture and propagation were studied on the three Hosta p lantaginea Var 'Emerald Tiara', 'Sweety' ,and'France', cultured by bud explants, results indicated that induction medium with MS+BA 5 mg/L+NAA 0.2 mg/L and subculture medium with MS+6-BA 2 mg/L+NAA 0.5 mg/L were the optimum medium. The best concentration of NAA was 0.1 mg/L for rooting. Plantlets transplanted on media which contained band and perlite with 4:1 had survival rate of more than 95%.
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