大鼠脑挫伤后巢蛋白表达与脑挫伤经过时间的关系  

Relationship between Expression of Nestin in Experimental Brain Contusion and Injury Time

作　　者：贾冬梅[1] 何光龙[1] 周亦武[1] 邓伟年[1] 孙来晶[1] 戴甲培[2] 刘良[1] 徐小虎[3] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]华中科技大学同济医学院神经生物学教研室,湖北武汉430030 [3]汕头大学医学院法医学教研室,广东汕头515031

出　　处：《法医学杂志》2006年第3期161-164,F0002,共5页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30570629);中国博士后科学基金资助项目(2003034459);湖北省自然科学基金资助项目(2003ABA140)

摘　　要：目的观察大鼠脑挫伤后不同时间内巢蛋白(nestin)蛋白表达的变化,探讨其与脑损伤经过时间的关系。方法利用自由落体撞击大鼠脑挫伤模型,在脑挫伤后不同时间(0.5,6,12h和1,3,7,14,28d)观察nestin蛋白在伤侧皮质、海马齿状回及胼胝体表达情况。结果伤后0.5h伤侧皮质、海马齿状回及胼胝体nestin阳性细胞表达开始增强,7d达高峰,以后逐渐下降,28d伤侧皮质、海马齿状回恢复至正常组水平,伤侧胼胝体在伤后28d仍有弱表达。结论nestin免疫组化染色可以作为法医学推断早期脑损伤时间的指标之一;nestin可作为判断脑损伤是否存在及区分生前和死后脑损伤的标志。Objective To observe the alteration of nestin intervals in the experimental traumatic brain injury and investigate its relation to the injury intervals. Methods The rat brain contusion was conducted by falling impact injury. After various survival interval （0.5,6,12h and 1,3,7,14,28 d）, immunohistochemical SP method was used for observing the expression of nestin in the cortex, hippocampal dentate gyrus and the corpus callosum on injury side. Results Expression of nestin positive cells increased at 0.5 h and reached the maximum level in 7 d after brain contusion, then the expression decreased gradually. The intensity of nestin staining in the the cortex and the hippocampal dentate gyrus decreased to normal on 28 d. As to the corpus callosum of injury side it remained weak on 28 d. Conclusion The changes of nestin immunohistochemical staining can be used as an index for forensic estimation of early injury time.

关 键 词：脑挫伤 巢蛋白 损伤时间 免疫组织化学 

分 类 号：R187.5[医药卫生—流行病学] R735.2[医药卫生—公共卫生与预防医学]