赤芍中芍药苷提取溶剂的选择  

Effects of Different Solvents on the Extraction of Paeoniflorin from Radix Paeoniae Rubra

作　　者：路小平[1] 

机构地区：[1]河南财经学院医院,河南郑州45002

出　　处：《时珍国医国药》2006年第6期1017-1018,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的 探讨赤芍药苷提取的最佳溶剂。方法 分别用甲醇、75％乙醇、重蒸馏水提取赤药中芍药苷，用YWG-C18（250×4．6mm）色谱柱，以甲醇-0．05mol／L磷酸二氢钾溶液（32：68）为流动相测定芍药苷，检测波长为230nm。结果 在所试浓度（0．010-0．200μg／ul）范围内线性关系良好r=0．9998，n=5；以甲醇、75％乙醇、重蒸馏水作溶剂赤芍苷的含量分别为3．01％，3．09％，3．07％。结论结果表明，以甲醇、75％乙醇、重蒸馏水作溶剂提取赤芍中芍药苷的含量无显著性差异（P〉0．05）；使用蒸馏水作溶剂可避免甲醇较强的毒性且廉价；我们认为使用重蒸馏水做溶剂优于乙醇、甲醇。Objective To select the optimal solvent to extract paeoniflorin from Radix Paeoniae Rubra. Methods Paeoniflorin was extracted from Radix paeoniae Rubra with methanol,75% ethanol and redistilled water , respectively . Paeoniflorin was separated on a YWG- C18 （250 mm× 4.6 mm） using methanol ：0.05 mol/L KH2PO4 （32：68 ） as a mobile phase and determined at 230 nm. Results The linear curve was ideal within the tested range of 0.010 - 0.200 μg/μl（ r = 0. 9998, n = 5 ） ;the average content of paeoniflorin extracted with methanol ,75% ethanol and redistilled water was 3.01% ,3.09% ,3.07%, respectively. Conclusion There was no significant difference in extraction of paeoniflorin with these three solvents （P 〉 0.05 ）, whereas methanol is highly toxic, redistilled water is a low -priced and safe solvent, indicating ettraction with redistilled water is better.

关 键 词：赤芍 芍药苷 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]