三波长分光光度法测定多穗柯叶中黄酮的含量  

Determination of Flavonoids in Lithocarpus Polysachyus rehd Leaves by Three-wavelength spectrophotometry

作　　者：肖坤福[1] 

机构地区：[1]浙江省丽水市丽水学院,浙江丽水323000

出　　处：《时珍国医国药》2006年第6期980-981,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的 清除由于多穗柯叶中黄酮光谱吸收峰不对称给定量分析造成的影响。方法 采用三波长分光光度法测定多穗柯叶中黄酮含量。结果 回归方程为△A=0．0384C＋0．0013；相关系数R=0．9995；检测波长λ1=460nm，λ2=420nm，λ3=380nm，线性范围为500～3000λg／ml，回收率为96．6％～106．2％，变异系数小于0．43％。结论 该方法的准确度和精密度均令人满意，且操作简便易行。Objective To solve the infection of asymmetric absorption peak in determination of flavonoids in lithocarpus polysachyus rehd leaves. Methods Determination of flavonoids in lithocarpus polysctchyus rehd leaves by three - wavelength spectrophotometry. Results Equation of linear regression is A =0.038 4C ＋0.001 3 ; measuration wavelength is λ1 =460 nm, λ2. =420 nm, λ3 = 380 nm, proportional band is 500 - 3 000 μg/ml, the recovery is 96.6% - 106.2% and the coefficient of variation is 0.43%. Conclusion Accuracy and precision of this method are turn up trumps and the operation is simple.

关 键 词：:黄酮类化合物 多穗柯 三波长分光光度法 

分 类 号：R284.2[医药卫生—中药学]