H^+对非洲爪蟾卵母细胞ATP-激活电流的调制作用  

Modulation of ATP-activated Current by H^+ in Xenopus Oocytes

作　　者：王照宇[1] 程剑侠[1] 朱凡[1] 张玉芹[1] 

机构地区：[1]武汉科技大学医学院,湖北武汉430080

出　　处：《武汉科技大学学报》2006年第3期303-305,共3页Journal of Wuhan University of Science and Technology

基　　金：湖北省自然科学基金资助项目(2004ABA152)

摘　　要：研究H+对非洲爪蟾卵母细胞内源性ATP受体的调制作用。采用双极电压钳技术记录卵母细胞膜ATP受体介导的电流。实验检测的细胞全部对ATP敏感,应用pH为7.4的ATP(10-3mol/L)后引起一特征性电流,呈浓度依赖性。改用pH为6.5,6.0,5.5的ATP(10-3mol/L)后,电流幅值明显减弱。结果表明,pH值降低,可抑制ATP诱导的卵母细胞膜电流。To investigate the modulatory effect of H ^＋ on ATP-activated current and the allosteric modulation through ATP receptor, double electrode voltage clamp technique was used to record the ATP-activated current in healthy Xenopus oocytes. The experimental results show that all the healthy oocytes examined （30/30） respond to ATP of pH 7.4 with an inward current in a dose-dependent （ 10^-3 mol/L） manner. It has been proved that the ATP-activated membrane current is suppressed by acid pH and alkaline pH.

关 键 词：H^+ 卵母细胞 ATP受体 双极电压钳技术 

分 类 号：R338.1[医药卫生—人体生理学]