硒与金属硫蛋白对小鼠肝损伤的防护作用研究  

Metallothionein Induced by Selenium and the Protection on Mice Liver Injury of Selenium-Metallothionein

作　　者：田晓丽[1] 唐欣[1] 左刚[2] 毛建平[2] 郭军华[1] 

机构地区：[1]中国人民解放军军事医学科学院附属医院临床药理室,北京100850 [2]中国人民解放军军事医学科学院放射医学研究所免疫室,北京100850

出　　处：《中国生物工程杂志》2006年第6期23-29,共7页China Biotechnology

基　　金：国家自然科学基金资助项目(30371664)

摘　　要：应用蛋白质芯片和RT-PCR技术研究硒对小鼠肝脏金属硫蛋白(Metallothionein,MT)的诱导表达;以蛋白质芯片技术为主要实验手段研究CCl4诱导的小鼠肝脏损伤后血清及肝脏蛋白质图谱变化,寻找小鼠血清及肝脏组织损伤标志物,同时观察有机硒(麦硒康,Organ-Se)对损伤的防护作用,结果表明与对照组相比给予有机硒后的小鼠肝脏MT表达明显;比较肝损伤组及用药组(Organ-Se)的血清及组织蛋白质表达图谱变化,发现血清中存在3个具有统计学意义的标志物:5062.5Da、5566.5Da、6358.5Da;肝脏组织中有4个具有统计学意义的标志物:5449.6Da、7131·5Da、9903.2Da和10767.3Da;与损伤组相比预先保护组(Organ-Se)的血清及肝脏标志物的表达水平接近正常组。实验表明非金属元素硒,尤其是有机硒同金属元素(如锌)一样,能够有效诱导MT的表达,为今后进一步开展硒代金属硫蛋白的机制研究奠定了基础;同时在对小鼠肝损伤保护的研究中发现,有机硒具有明显的肝损伤保护作用,是一种较有前途的值得开发的肝损伤防护药。Using Proteinchip and RT-PCR to verify the induction of metallothionein by selenium. Results showed there was definite effect of metallothionein induced by selenium, and indicated the protection of mice liver injury by selenium , the biomarker of liver injury had also been found. The effect induced by organic selenium （Selenium melt） is stronger than that by inorganic selenium （Na2SeO3 ） and better or nearly equal to ZnSO4 which is accepted and used by popular as a good one of the synthesis which can induce MT; The protein expression difference of mice serum and liver among control group, injury group and pre-treat group was found by Using SELDI-TOF-MS, three biomarker had be found in serum on WCX proteinchip：5062.5Da , 5566.5Da, 6358.5Da;In liver tissue there are four biomarker ：5449.6Da, 7131.5Da, 9903.2Da and 10767.3Da found on WCX proteinchip. The experiments demonstrated Selenium, special organic selenium （Selenium melt） be a strong and novel MT in the future, and can protect the liver injury by selenium.

关 键 词：硒 金属硫蛋白 蛋白质芯片 肝损伤防护 

分 类 号：Q513[生物学—生物化学] S816.72[农业科学—饲料科学]