脂蟾毒配基诱导人肝癌细胞凋亡作用的研究  

The Investigation of the Effect on Human Hepatoma Cell Apoptosis Induced by Resibufogenin

作　　者：肖义秀[1] 张瑾峰[2] 吴白燕[3] 

机构地区：[1]中国科学院研究生院,北京100049 [2]首都医科大学细胞生物学系,北京100069 [3]北京大学医学部医学遗传学系,北京100083

出　　处：《中国生物工程杂志》2006年第6期36-39,共4页China Biotechnology

摘　　要：目的探讨脂蟾毒配基对体外培养人肝癌细胞(Bel7402)的诱导凋亡作用,为研究其对肿瘤细胞生长抑制作用的机制提供依据。方法应用流式细胞光度术检测细胞凋亡;采用细胞免疫细胞化学显色检测和Westernblotting分析凋亡相关基因蛋白的表达来研究脂蟾毒配基对人肝癌细胞(Bel7402)凋亡的诱导作用。结果表明脂蟾毒配基能够诱导Bel7402细胞发生凋亡,凋亡率大于50%;脂蟾毒配基提取液(浓度1.0μm)作用于Bel7402细胞24h后,bc1-2蛋白的表达下调,到48h、72h后下调更明显;而Bax蛋白的表达从24h后开始上调,到48h、72h后表达上调明显。使用方差分析法与对照组相比较,P<0.05,统计学有显著意义。结论提示诱导肿瘤细胞凋亡可能是脂蟾毒配基抑制、杀伤人肝癌细胞的机制之一。Objective ： To study the mechanism of resibufogenin inhibiting on the growth of human hepatoma cell by analyzing the apoptosis induced by resibufogenin. Methods The apoptosis was observed by means of Flow Cytometry. The effects of resibufogenin on DNA synthesis and expression of correlative proteins in hepatoma cell apoptosis was observed by means of Western blotting and cytochemical method. Result It was showed that resibufogenin could induce Be17402 cell （human hepatoma cell） to apoptosis. The rate of apoptosis exceeds 50 percent. The expression of inhibiting apoptosis gene bcl-2 decrease after 24 hours in the group of 1.0 resibufogenin and decreased more significantly after 48 and 72 hours; the expression of promoting apoptosis gene Bax increased after 24 hours and increased more significantly after 48 and 72 hours. Conclusion The result indicate that inducing neoplasm cell apoptosis may be one mechanism of the resibufogenin inhibition and kill of the human hepatoma cell.

关 键 词：脂蟾毒配基 流式细胞光度术 人肝癌细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学]