^(60)Coγ射线对高免卵黄抗体(IgY)活性和理化特性的影响  

Effect of ^(60)Co γ Ray on Activity and Physic chemistry Characters of Intensely Immunized Egg Yolk(IgY)

作　　者：霍乃蕊[1] 柴桂珍 郑明学[3] 韩克光[3] 

机构地区：[1]山西农业大学食品科学与工程学院 [2]山西省兽药检测所,山西太原030000 [3]山西农业大学动物科技学院,山西太谷030801

出　　处：《激光生物学报》2006年第3期278-282,共5页Acta Laser Biology Sinica

基　　金：山西省科委攻关项目(971015);山西农业大学科技创新项目(2004023)

摘　　要：利用60Coγ射线照射卵黄抗体,分析了60Coγ射线对IgY的效价、纯度、热稳定性、耐酸性以及酶水解试验等理化特性的影响和不同温度下的保存时间。结果表明照射达10 kGy时,对IgY的效价和纯度无影响;对照组和照射组热稳定性、耐酸性无差异,但照射组更易被酶水解;照射后的IgY明显延长了保存期。The Immunized Egg Yolk was irradiated by ^60Coγ ray, and then we investigated the effect of ^60Coγ ray on antibody titer,purity,heat stability,acid resistance,enzymatic hydrolysis and other activity and physic-chemistry characters of it. When irradiation was up to 10 kGy, the results showed that there was no effect on antibody titer and purity of IgY; there was no differece in heat stability and acid resistance beween the irradiated group and the control group, but the irradiated group was more easely hydrolyzed by enzyme ;The retention period of irradiated IgY was obviously prolonged.

关 键 词：^60Coγ射线 高免卵黄抗体 理化特性 

分 类 号：Q945.4[生物学—植物学] Q6-33