黄芪总提物对氧化应激致肝星状细胞增殖与合成胶原的抑制作用及其机制  

The inhibitory effect of total astragalus extract on HSC-T6 cell proliferation and collagen synthesization induced by oxidative stress

作　　者：徐明[1] 朱虹[1] 吴樱樱[1] 邹宇宏[1] 姚余友[1] 杨雁[1] 

机构地区：[1]安徽医科大学药理教研室,合肥230032

出　　处：《中药药理与临床》2006年第3期60-63,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：安徽省教委课题基金资助项目(编号:2002kj152)

摘　　要：目的：研究黄芪总提物（TAE）体外对氧化应激致HSC-T6细胞增殖与合成胶原的抑制作用及其机制。方法：用四甲基偶氮唑（MTT）比色法检测HSC-T6细胞增殖；碱水解法、硫代巴比妥酸（TBA）法和黄嘌呤氧化酶法分别检测羟脯氨酸（HyP）、MDA和SOD；荧光分光光度法检测Fe^2＋-H2O2反应产生羟自由基（·OH），紫外分光光度法检测黄嘌呤-黄嘌呤氧化酶体系和非酶体系产生超氧阴离子O2^-）。结果：12．5～100mg／L TAE对低浓度Fe^2＋-H2O2诱导HSC-T6细胞增殖以及胶原产生有显著的浓度依赖性抑制作用，并可降低MDA含量和升高SOD活性。6．25～100mg／L TAE可浓度依赖性地抑制Fe^2＋-H2O2体系产生·OH；还可对黄嘌呤（X）-黄嘌呤氧化酶（XO）体系以及非酶体系产生O2^-均有显著的抑制作用，而对黄嘌呤氧化酶活性的抑制作用较弱。结论：TAE可明显抑制氧化应激致HSC-T6细胞的增殖与合成胶原，该效应可能与其直接清除·OH和O2^-自由基以及提高SOD酶活性有关。Aim： Study the inhibitory effect of total astragalus extract（TAE） in vitro on HSC-T6 cell proliferation and collagen synthesization induced by oxidative stress and its mechanism. Methods： The proliferation of HSC-T6 cell was examined by MTT colorimetric. The methods of Alkali hydrolyzation, thiobarbituric acid （TBA） and xanthine oxidase were respectively applied to determine the levels of hydroxy proline （HyP）, maldondialdehyde（MDA） and superoxide dismutase（SOD）; The levels of the hydroxyl radical（·OH） generated in Fe^2＋-H2O2 system and superoxide anion （O2^-） generated in xanthine-xanthine oxidase or non-enzyme systems were respectively detected by fluorescence spectrophotometry and ultraviolet spectrophotometry. Results： TAE（12.5～100 mg/L）could concentration-dependently inhibit HSC-T6 cell proliferation and collagen synthesization in the presence of low concentration of Fe^2＋-H2O2, and decrease MDA content and increase SOD activity. Further study showed that TAE （6.25～100 mg/L）could concentration-dependently inhibited Fe^2＋-H2O2 produced·OH, and markedly inhibit the hydroxylation of benzoic acid induced by Fenton reaction generated hydroxyl radical. Moreover, TAE could inhibit NBT reduction induced by both xanthine-xanthine oxidase and non-enzyme generated superoxide anion, but had no remarkable effect on the activity of xanthine oxidase, suggesting that TAE could have the scavenging effect on O2^-. Conclusion： All the result suggested TAE could remarkably inhibit HSC-T6 cell proliferation and collagen synthesization induced by oxidative stress. These effects may be related to its antioxidative activity.

关 键 词：黄芪总提物 肝星状细胞 羟脯氨酸 羟自由基 丙二醛 

分 类 号：R285[医药卫生—中药学]