灵芪蠲肝液对急性肝损伤NO和氧自由基的影响  

The Effect of Lingqijuangan Liquor(灵芪蠲肝液) on NO and oxygen free radicals on acute hepatic injury

作　　者：李万平[1] 李晓冰[1] 李华[1] 肖顺汉[1] 邓存良[2] 陈枫[2] 唐坤华[2] 

机构地区：[1]四川泸州医学院药理教研室 [2]泸州医学院附属医院传染免疫研究室,泸州646000

出　　处：《中药药理与临床》2006年第3期153-154,共2页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:研究灵芪蠲肝液对急性肝损伤的保护作用机制。方法:用硫代乙酰胺复制大鼠急性肝损伤模型,检测肝功能、一氧化氮(NO)含量以及肝组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)水平并取肝组织做病理检查。结果:硫代乙酰胺急性肝损伤时,灵芪蠲肝液能使血清ALT、AST活性、TB il、MDA、NO水平明显降低,升高SOD水平;病理检查发现其能减轻肝细胞变性、坏死,减少炎细胞浸润。结论:灵芪蠲肝液通过减少氧自由基的产生,减少NO的产生,从而保护肝细胞膜。Objective： To observe the mechanism of Lingqijuangan Liquor（LQL） on acute hepatic injury. Methods： Acute hepatic injury was induced by thioacetamide. Then hepatic functions and the levels of serum nitric oxide （NO） and 10% liver homogenates＇ malonyldialdehyde （MDA） and superoxide dismutuse（SOD） were detected. Take liver tissue to do pathological examination. Results： Compaired with model group, blood serum ALT and AST activities, TBil and NO levels and MDA levels in homogenates were significantly decreased by LQL; and SOD levels were obviously increased by LQL when livers were acute injuried. Pathological examination found that LQL can lighten hepatic cell denaturalization and putrescence and reduce inflammation cell infiltration. Conclusion： LQL can lighten the thioacetamide induced acute liver injury by decreasing production of oxygen free radicals and reducing production of NO so as to protect liver cellular membrane.

关 键 词：灵芪蠲肝液 急性肝损伤 氧自由基 一氧化氮 

分 类 号：R285[医药卫生—中药学]