阳极损毁海马腹侧下托可减少成瘾大鼠的条件位置偏爱  

Electrolytic lesions of ventral subiculum to reduce conditioned place preference in drug addicted rats

作　　者：王建[1] 宋乐[1] 胡三觉[1] 

机构地区：[1]第四军医大学全军神经科学研究所,陕西西安710033

出　　处：《第四军医大学学报》2006年第12期1083-1085,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金重点项目30530260

摘　　要：目的:研究损毁腹侧海马下托(VSUB)是否能够阻止成瘾大鼠的条件位置偏爱.方法:雌性SD大鼠,经过吗啡强化训练使其形成条件位置偏爱(CPP).CPP形成并稳定之后,将大鼠分成3组:损毁组20只,接受双侧VSUB的直流电损毁;假手术组10只,接受与手术组相同的手术操作,但不通电流;空白对照组10只,不作任何处理.经过5d的恢复,3组大鼠再次测定CPP时间,确定其药物渴求的程度.结果:行为学测试结束后,将样本灌注切片进行组织学评定,损毁组脑片组织缺损位置局限于VSUB区域.损毁组大鼠伴药箱停留时间明显减少[(93.1±26.0)s],相对于假手术组[(10.1±4.5)s]和空白对照组[(5.1±4.1)s]的大鼠在伴药侧停留的时间减少量有显著性差异(P<0.05).结论:VSUB在大鼠对药物的渴求中起着非常重要的作用,损毁该部位可以阻止成瘾大鼠的条件位置偏爱.AIM： To investigate whether the ventral subiculum （VSUB） lesions can release the drug craving from drug addicted rats. METHODS： Female SD rats were trained to acquire the morphine conditioned place preference （CPP）, After the preference was established, the rats were divided to 3 groups, The lesion group （ n = 20） received the electrolytic lesions of VSUB, the sham lesion group （ n = 10） received the same operation to the lesion group but no current passed, and the control group （ n = 10） received no operation. After 5 d recovery, the 3 groups underwent the CPP testing again to observe the extent of drug craving, RESULTS： A histological test was performed on postlesion rats after a behavioral test, and the lesions were found confined to the VSUB. The time that the lesion group spent in the drug-paired side was significant decreased [ （93. 1 ± 26.0） s ] compared with both sham group [ （ 10. 1 ± 4. 5 ） s ] and control group [（5. 1 ±4.1） s]. （P〈0.05）. CONCLUSION： The VSUB play an important role in drug addiction and lesions of the exact part can release the drug craving from drug addicted rats.

关 键 词：条件位置偏爱 海马 电解损毁 吗啡依赖 药物渴求 

分 类 号：Q189[生物学—神经生物学] Q55[生物学—普通生物学]