小鼠附植前胚胎培养基的优化  

Optimization of Mouse Preimplantation Embryo Culture Media

作　　者：高淑敏[1] 温晓辉[1] 邸科前[1] 魏巍[2] 李相运[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]北京大学生命科学学院,北京100871

出　　处：《西北农业学报》2006年第4期6-9,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(编号:30571336);河北农业大学校长基金资助

摘　　要：从4种常用培养基M16、MTF、CZB、KSOM中筛选适合CD-1小鼠附植前胚胎发育的体外培养基,并分析磷酸二氢钾、乳酸钠、葡萄糖、牛磺酸以及必需与非必需氨基酸对CD-1小鼠胚胎体外培养的影响。发现KSOM的培养效果明显优于其他培养基。在KSOM中添加必需氨基酸对8-细胞以前的胚胎发育有抑制作用,但添加牛磺酸、必需氨基酸与非必需氨基酸都可提高囊胚的细胞数。优化后的分阶段KSOM培养基显著提高了早期胚胎的囊胚发育率和囊胚期细胞数目。To develop an effective CD-1 mouse embryo culture medium, we evaluated the effects of several culture media including M16, MTF, CZB, KSOM and taurine, essential amino acids and nonessential amino acids were added in KSOM medium to observe their effects on the development of mouse preimplantation embryos. The KSOM was an outstanding one among the media listed. Adding taurine, essential amino acids and nonessential amino acids in KSOM medium can increase the ceil , number of CD-1 mouse morula and blastocysts, however, adding essential amino acids in KSOM media retarded the development rate of early CD-1 mouse embryos. Staged KSOM media K1/K2 （K1 for pre-8-cell stage embryos containing 0. 175 mM KH2PO4, 20 mM sodium lactate, 0. lmM glucose and 0.1mM taurine, K2 for post-8-cell stage embryos containing 0. 35 mM KH2PO4, 5mM sodium lactate, 5.56 mM glucose, 0.1 mM taurine, 0.25 mL 100× nonessential amino acids and 0.5 mL 50× essential amino acids） were formed by changing the dose of sodium lactate, KH2PO4, glucose, taufine, essential amino acids and nonessential amino acids. The optimized K1/K2 media could significantly improve the blastocysts development rate and the cell number of morula and blastocysts.

关 键 词：CD-1小鼠 KSOM 体外培养 

分 类 号：Q813.7[生物学—生物工程]