SARS-CoV感染猴模型病毒学研究  

The SARS virus isolation from monkeys infected with SARS-CoV

作　　者：蒋虹[1] 鲍琳琳[1] 涂新明[1] 丛喆[1] 佟巍[1] 高虹[1] 朱华[1] 魏强[1] 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所,北京100021

出　　处：《医学动物防制》2006年第6期409-411,共3页Journal of Medical Pest Control

基　　金：863课题;编号:2003AA2082052003AA208213

摘　　要：建立SARS-CoV病毒分离培养方法,确定SARS-CoV病毒感染动物后在不同时间和不同组织病毒的复制和存活时间,并比较中国恒河猴和食蟹猴对病毒的敏感性,筛选出对SARS冠状病毒敏感的动物,完善SARS冠状病毒感染猴模型的病毒学指标。方法:SARS-CoV经鼻腔接种实验用猴,定期采集动物的鼻咽拭子、血浆和组织标本进行病毒分离培养,分离出来的病毒用免疫荧光方法和中和试验等方法进行测定。结果:SARS冠状病毒均可感染恒河猴和食蟹猴,在鼻咽拭子、血浆和组织中可以分离到病毒。结论恒河猴和食蟹猴均可作为SARS感染动物模型,而病毒分离培养可以作为感染模型的重要指标。Objective：To establish the method for isolation of SARS-coronavirus （SARS-CoV）, try to confirm whether rhesus or cynomolgus macaques could be infected with SARS-CoV, and determine the distribution and duration of the virus animals as SARS model. Method The monkeys were infected with identified SARS-CoV isolated from human SARS patient. The swab and serum samples were collected at 4, 7, 10 days after inoculation. Tissues were collected at different time. All samples were cultured with Veto-E6 cells. Result The SARS-CoV was detected from various samples from infected animals. Conclusion Viral isolation is an unreplaced method for testing virus replication in Vivo

关 键 词：SARS冠状病毒 动物模型 病毒分离培养 

分 类 号：R392.11[医药卫生—免疫学] R512.6[医药卫生—基础医学]