核桃楸总黄酮及胡桃醌含量测定  

Extraction and Determination of Total Flavonoid and Juglone in Juglans mandshurica Maxim.

作　　者：孙墨珑[1] 宋湛谦 方桂珍[4] 

机构地区：[1]东北林业大学理学院,黑龙江哈尔滨150040 [2]中国林业科学研究院林产化学工业研究所 [3]国家林业局林产化学工程重点开放性实验室,江苏南京210042 [4]东北林业大学材料科学与工程学院,黑龙江哈尔滨150040

出　　处：《林产化学与工业》2006年第2期93-95,共3页Chemistry and Industry of Forest Products

基　　金：黑龙江省自然科学基金资助项目(C2004-28)

摘　　要：研究采取常规提取法、索氏提取法和超声波提取法浸提核桃楸样品(核桃楸的树叶、树皮和外果皮)。采用紫外-可见分光光度法测定核桃楸样品总黄酮含量,高效液相色谱法测定核桃楸样品胡桃醌含量。研究结果表明,总黄酮含量和胡桃醌含量分别为超声波提取法>常规提取法>索氏提取法。3种提取法浸提核桃楸样品,总黄酮含量均有树叶>树皮>外果皮的趋势;胡桃醌含量均有外果皮>树叶>树皮的趋势。超声波法提取核桃楸样品总黄酮含量分别为:树叶5.00%,树皮4.17%,外果皮3.05%;胡桃醌含量分别为:树叶0.002 09%,树皮0.001 31%,外果皮0.002 29%。The extractions of total flavonoid and juglone ffrom leaves, bark and green peel of Juglans mandshurlca Maxim. trees were carried out by three extracting methods respectively, including common method, reflux method and ultrasonic method. UV spectroscopy was used for determining the amount of total flavonoid, and HPLC was used for determining the amount of juglone. Results showed that amounts of total flavonoid and juglone are in the order of： ultrasonic method 〉 common method 〉 reflux method. Furthermore amounts of total flavonoid extracted from three materials by all three method showed the order of leaves 〉 bark 〉 green peel. Amounts of juglone extracted from three materials by all three method showed the order of： green peel 〉 leaves 〉 bark. The amounts of total flavonoid and juglone extracted by ultrasonic method are ： 5.00 % and 0. 002 09 % for leaves, 4.17 % and 0.001 31% for bark, 3.05 % and 0.002 29 % for green peel, respectively.

关 键 词：核桃楸 总黄酮 胡桃醌 

分 类 号：TQ351.014[化学工程]