非创伤性双下肢缺血后处理对大鼠I/R心肌梗死面积和PKCα表达的影响  

Effects of Non-wounded Leg Ischemic Postconditioning on Sizes of Myocardial Infarction Induced by Ischemia/reperfusion and PKCα Expression in Rats

作　　者：袁志柳[1] 王艺明[2] 沈正[1] 陈保林[3] 黄达枚[1] 刘兴德[1] 

机构地区：[1]贵阳医学院附院心内科 [2]贵阳医学院附院心理科,贵州贵阳550004 [3]贵州省人民医院,心内科550002

出　　处：《贵阳医学院学报》2006年第3期238-240,共3页Journal of Guiyang Medical College

摘　　要：目的:探讨在体条件下,非创伤性双下肢缺血后处理(N-W IPTC)对缺血-再灌注(I/R)心肌梗死面积(IS)和心肌组织中蛋白激酶Cα(PKCα)表达的影响。方法:采用SD大鼠心肌I/R模型,应用Evan’s蓝、TTC染色,观察N-W IPTC对IS的影响;运用免疫组化技术观察N-W IPTC对心肌组织PKCα表达的影响。结果:与I/R组比较,N-W IPTC组的IS显著降低(P<0.01或P<0.05);与I/R组比较,N-W IPTC组心肌细胞膜PKCα表达明显多于I/R组(P<0.01)。结论:N-W IPTC能有效降低I/R心肌的IS,其机制可能与促进心肌细胞膜PKCα的表达有关。Objective： To observe the protective effects of non-wounded leg ischemic postconditioning （N-WIPTC） on ischemia/reperfusion （I/R） myocardial injury in rats. Methods： 24 Sprague-Dawley rats weredivided randomly into four groups and the models of ischemia/reperfusion myocardial injury were produced. The infarction sizes （IS） were measured with Evan＇ s and Trc staining, and the expression of protein kinase C （PKCα） in myocardial tissue was investigated by immunohistochemistry. Results： Compared with those of I/R group, the ISs of N-WIPTC were lower （P 〈0. 01 or P 〈0. 05）, and the expression of PKCα was significantly higher in N-WIPTC groups （P 〈 0. 01 ）. Conclusions： N-WIPTC could significantly decrease the IS during I/R. The mechanism might be the promoted expression of PKCα in myocardial tissue after N-WIPTC.

关 键 词：心肌再灌注损伤 蛋白激酶C 心肌梗塞 大鼠 

分 类 号：R542.22[医药卫生—心血管疾病] R542.2[医药卫生—内科学]