人溶菌酶基因cDNA的克隆和序列分析  

Cloning and Sequencing of Human Lysozyme cDNA

作　　者：何晓宁[1] 彭树英[1] 杨瑞锋[1] 刘凤军[1] 郑月茂[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨陵712100

出　　处：《安徽农业科学》2006年第11期2333-2335,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"863"高技术资助项目(2001AA213081);西北农林科技大学人才基金资助项目

摘　　要：根据GenBank中的人溶菌酶(hLYZ)mRNA序列设计引物,以人胎盘组织总RNA为模板,通过RT-PCR方法扩增出长度为0.85 kb的hLYZ cDNA序列,经过序列测定表明所克隆的片段与已发表的hLYZ cDNA序列的同源性为99%。推导的氨基酸序列经blastp分析与人溶菌酶蛋白质前体的同源性为97%,成熟肽的同源性为99%,为进一步构建人溶菌酶基因乳腺特异性表达载体奠定了基础。According to the mRNA sequence of human Lysozyme （hLYZ） derived from Genbank, a pair of primers was designed. Human lysozyme （hLYZ） eDNA was amplified by RT-PCR from total RNA of human placenta which was 0.85 kb. The cloned fragment was sequencd and compared with hLYZ eDNA which was published, the result indicated that the sequence homology was 99%. Sequence analysis with blastp indicated that the deduced amino acid sequence has the homology of 97% and 99% respectively with the precursor protein of hLYZ and mature peptide. Which is the vital basis for the further construction of mammary gland-specific expression vector.

关 键 词：人溶茵酶 CDNA 克隆 序列分析 

分 类 号：Q786[生物学—分子生物学]