干细胞因子结合基质蛋白对毛囊无色素黑素细胞黏附与移行的调节作用  

Stem cell factor combined with matrix proteins promotes attachment and migration of amelanotic melanocyte from human follicle hair

作　　者：王大光[1] 朱文元[1] 马慧军[1] 岳学状[1] 李诚让[1] 

机构地区：[1]南京医科大学第一附属医院皮肤性病科,江苏南京210029

出　　处：《临床皮肤科杂志》2006年第7期423-426,共4页Journal of Clinical Dermatology

基　　金：国家自然科学基金资助项目(39870701和39570658)

摘　　要：目的:研究干细胞因子(stemcellfactor,SCF)结合基质蛋白对毛囊无色素黑素细胞(amelanoticmelanocytes,AMMC)黏附与移行的调节作用。方法:四甲基偶氮唑蓝(MTT)法测定细胞的黏附率。48孔细胞趋化小室测定细胞移行。使用罗丹明标记的鬼笔环肽标记肌动蛋白(F-actin),共聚焦显微镜观察SCF对细胞骨架的调节作用。结果:纤黏连蛋白(FN)、板层素(LN)和Ⅳ型胶原(CⅣ)均以浓度依赖方式促进了AMMC的黏附,其中FN和LN对AMMC促黏附作用相近。SCF减少了AMMC对FN和CⅣ的黏附,对FN的作用更为明显,而对LN起促黏附作用。FN和CⅣ对AMMC有趋化作用,LN无明显作用。SCF作用后可明显使FN对AMMC的趋化作用增强。SCF单独对AMMC有趋化作用,结合FN后,作用明显加强。未黏附在FN上的AMMC胞质内无明显应力纤维;黏附在FN上以后,胞质内可见F-actin排列规则并聚集成束,形成束状应力纤维;SCF作用后,胞体内应力纤维更加致密。结论:SCF调节了AMMC在基质蛋白FN、LN和CⅣ上的黏附与移行,尤其是SCF与FN具有协同促移行作用这可能是白癜风复色过程中AMMC从外毛根鞘向表皮移行机制的一部分。Objective： To investigate the effects of stem cell factor （SCF） combined with matrix protein on attachment and migration of amelanotic melanocyte （AMMC） from human follicle hair. Methods： Cell attachment assays were carried out using 96-well plate pre-coated with different concentrations of matrix proteins of fibronectin （FN）, laminin （LN） and type Ⅳ collagen （CⅣ）. The attachment rate was measured by MTT method. The effects of matrix protein and SCF on AMMC chemotaxis were tested by Neuro Probe standard 48 wells chemotaxis chamber. F-actin was labeled by rhodamine-conjugated phalloidin, then organization of the actin cytoskeleton was observed by confocal microscope. Results： FN, LN and CIV promoted attachment of AMMC in concentration-dependent manner. FN had the same effect as LN, and C Ⅳ showed the weaker potency than the others. SCF decreased the effect of FN and CⅣ on promoting AMMC attachment in dose-dependent manner, however, the effect of LN was increased. LN had no chemotaxis effects on AMMC, while FN and CⅣ had chemotactic effects on AMMC. SCF could clearly increased the chemotactic effect of FN. SCF showed the chemotactic effect on AMMC alone, which was promoted by FN. FN promoted AMMC to express stress fibers arranged in regular manner. The density and thickness of stress fibers were clearly increased after treatment of SCF. Conclusion： SCF regulates the interaction between AMMC and matrix proteins. SCF combined with FN and CIV promotes migration of AMMC, which may interpret partly the mechanism of migraton of AMMC from hair-follicle outer-root-sheath into epidermis during vitiligo repigmentation.

关 键 词：黑素细胞 无色素 毛囊 干细胞因子 基质蛋白 纤黏连蛋白 

分 类 号：R361.2[医药卫生—病理学]