免疫酶组织化学法检测红斑狼疮皮损狼疮带研究  

Study of lupus band test in lesions of lupus erythematosus by immunohistochemical method

作　　者：严煜林[1] 由蕾[1] 罗虹[1] 

机构地区：[1]广西医科大学第一附属医院皮肤性病科,广西南宁530021

出　　处：《临床皮肤科杂志》2006年第7期443-445,共3页Journal of Clinical Dermatology

摘　　要：目的:探讨用免疫组化方法检测红斑狼疮皮损中的免疫球蛋白沉积对其诊断的价值。方法:用辣根过氧化物酶(HRP)标记抗体法分析研究387例红斑狼疮患者皮损中免疫球蛋白(IgG、IgA、IgM)及补体(C3、C1q)的沉积情况以及与临床诊断、组织病理学诊断的符合率。结果:临床诊断为红斑狼疮,且组织病理学诊断亦符合红斑狼疮的患者皮损中最常见、最易检出的狼疮带成分均为IgG、IgM,其次为C1q,C3与IgA不易检出;而临床诊断为红斑狼疮,但组织病理学诊断不符合的患者,皮损中几种免疫球蛋白和补体的检出率之间尚无明确差别。结论:免疫组化法检测狼疮带对红斑狼疮患者的临床和组织病理学诊断有重要参考价值,且此法简便易行,具有较好的可重复性。Objective： To explore the immunoglobulin deposit in lesions of lupus erythematosus（LE） by immunohistochemical method and its significance in clinical diagnosis. Methods： The deposition of Immunoglobulin （IgG, IgA, IgM） and complement （C3, Clq） was examined in lesions of 387 LE patients by lupus band test （LBT） using immunopathologic technique horseradish peroxidase. After the examination, the coincidence between clinical diagnosis and histopathologic diagnosis was analyzed statistically. Results： IgG and IgM were the very common deposition in clinical LE patients and histopathologic LE patients. C3 and IgA deposit were not common. For those LE patients with clinical diagnosis standards but no typical features in histopathology, there was no difference between the detection rate of the immunoglobulins and complement. Conclusions.＂ LBT detected by immunohistochemical method is significant and very helpful to clinical and pathologic diagnosis for LE patients. This technique is convenient to carry out and is reliable.

关 键 词：狼疮 红斑 免疫酶组织化学法 狼疮带试验 免疫球蛋白 

分 类 号：R593.24[医药卫生—内科学]