采用环氧化合物交联牛颈静脉带瓣管道的实验研究  

Bovine jugular venous conduit treated with the polyepoxy compound

作　　者：徐朝军[1] 吴忠仕[1] 胡铁辉[1] 胡建国[1] 冯耀光[1] 马忠厦[1] 王晖[1] 胡剑[1] 胡野荣[1] 

机构地区：[1]中南大学湘雅二医院心胸外科,长沙410011

出　　处：《中南大学学报（医学版）》2006年第3期355-358,共4页Journal of Central South University ：Medical Science

基　　金：湖南省科技厅科技计划重大专项基金(04sk1005)

摘　　要：目的:探讨采用环氧化合物交联新型肺动脉血管替代物之一牛颈静脉带瓣管道(BJVC)的方法和效果。方法:新鲜BJVC24根,随机分为环氧化合物处理组(PC组,n=8),戊二醛处理组(GA组,n=8)及新鲜对照组(n=8)。分别测量各组处理前后管壁厚度、管腔外径及瓣膜的抗返流功能的变化。测定处理前后各组的组织含水量、热皱缩温度、组织断裂强度及断裂伸长率。另外进行大鼠皮下埋植试验,60d后测定组织钙含量。结果:PC组外观形态、管腔外径、瓣膜抗返流功能更接近天然血管;PC组及GA组机械性能相当,且明显强于新鲜对照组(P<0.05);大鼠皮下包埋后PC组钙含量显著低于GA组(P<0.05)。结论:PC交联BJVC可有效保持组织的柔软性,保护其瓣膜的抗返流性能,且具备良好的抗钙化性能,是一种有效的合适的生物组织处理制备的新型技术。Objective To determine the feasibility whether the bovine jugular venous conduit （BJVC） can be fixed with polyepoxy compound （PC）. Methods Twenty-four BJVCs were divided into 3 groups and fixed with polyepoxy compound （PC group, n = 8 ）, glutaraldehyde （GA group, n = 8 ）, and unfixed group （Control group, n = 8 ） , respectively. The morphologic and mechanical properties of BJVCs in the 3 groups, including thickness, diameter, moisture content, denaturation temperature, tensile strength, elongation at break, and fixation index were measured. The rat subcutaneous model for the assessment of tissue calcification was used. The calcium content in bovine jugular vein patches and valves was determined by flame atomic absorption spectrophotometer. Results There was no difference in the wall thickness, diameter, and tissue water content between PC and the control group, but significant difference was found between GA and PC groups. The mechanical pro- perties of PC group and GA group were not significantly different, but they were better than those of the control group. GA-fixed BJVC samples showed clear calcification, while PC fixed BJVC were cal- cified significantly less. Conclusion PC is an effective and suitable choice for the treatment of BJVC since it can effectively preserve the structure and the anti-reflow function of valves in bovine jugular vein and it has better anti-calcification properties.

关 键 词：牛 颈静脉 环氧化合物 交联 

分 类 号：R654.2[医药卫生—外科学]