拉坦前列素对体外培养的猪毛囊生长的影响  

Effect of latanoprost on pig hair follicles in vitro

作　　者：张卉[1] 李晓凤[1] 范卫新[1] 

机构地区：[1]南京医科大学第一附属医院(江苏省人民医院)皮肤科,210029

出　　处：《中华皮肤科杂志》2006年第7期391-393,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金(30170860)

摘　　要：目的探讨拉坦前列素对体外培养的猪毛囊生物学特性的影响。方法采用猪毛囊体外培养模型,记录体外培养的猪毛囊生长速度和毛球部形态学改变,评价拉坦前列素对毛发生长的作用。结果与阴性对照组比较,拉坦前列素质量浓度达到0．8ng/mL时即能使培养的猪毛囊生长速度加快(P＜0．05),并持续生长达8 d,且毛囊毛球部仍然呈现生长期样形态,而对照组则表现为退行期／休止期样外观。结论拉坦前列素对体外培养的猪毛囊生长具有明显的促进作用。能够延长毛囊的生长期。Objective To investigate the effect of latanoprost on the growth of pig hair follicles in vitro. Methods A culture model of pig hair follicles was applied. The morphological changes in the hair bulbs and growth speed of the hair follicles cultured with different concentrations （ 0.032, 0.16, 0.8, 4, 20, 100, 500, 2 500 ng/mL ） of latanoprost were observed and recorded on day 1, 2, 4, 6, 8 in the culture. Results Compared with the follicles without latanoprost, those cultured with the drug （ 0.8, 4, 20 ng/mL ） grew more rapidly （ P〈 0.05 ）. And on day 8 the follicular bulbs treated with latanoprost （ 0.8, 4, 20 ng/mL ） were still in an anagen-phase, while those without the drug exhibited a catagen/telogen-phase appearance. Conclusion The study shows that certain concentrations of latanprost could stimulate the growth of cul- tured pig hair follicles, which may be due to prolonging of the anagen phase in hair cycle.

关 键 词：毛囊 组织培养技术 拉坦前列素 米诺地尔 生长调节素类 

分 类 号：R96[医药卫生—药理学]