应用预制型无机诱导因子复合性工程支架材料行山羊组织工程化骨构建的放射性核素显像监测  

Radionuclide observation of repairing goat mandible defects with prefabricated-mineral inducing bone tissue engineered grafts

作　　者：康然刚[1] 王来平[1] 刘雨生[2] 周来生[3] 戴云海[4] 李容新[1] 陈梅梅[1] 张志宏[1] 周媛[1] 叶茂昌[1] 

机构地区：[1]安徽省立医院口腔颌面外科,合肥230001 [2]安徽省立医院生殖医学中心,合肥230001 [3]皖南医学院弋矶山医院核医学科,芜湖241000 [4]美国波士顿大学分子生物相容学研究室,波士顿021182394

出　　处：《口腔医学》2006年第3期211-213,共3页Stomatology

基　　金：安徽省重点科研项目(03021009);安徽省国际科技合作重点项目(04088009)

摘　　要：目的探讨预制型无机诱导因子复合性工程支架材料在骨大型缺损中的修复能力,及放射性核素显像技术在此过程中的应用价值。方法实验山羊15只按第4、8、12周时间点分为3组。采用自身配对设计,左侧置入支架材料,右侧空白对照。术后4、8、12周分别通过放射性核素99mTc-MDP骨显像进行监测。结果放射性核素骨显像中感兴趣区(ROI)计数和摄取比值显示:对照组在3个时间点均未见明显核浓聚性的再血管化及明显成骨现象,而实验组则随时间呈单驼峰状,在8周时达峰值,而到12周时血管化已完成,转为以成骨为主,故出现ROI和摄取比值的轻度下降趋势。结论99mTc-MDP监测到新生骨的良好成骨效能和再血管化状况,是一种较好的骨组织工程构建骨的监测手段。Objective To investigate the effects of prefabricated - mineral inducing bone tissue engineered grafts on repairing large defects in goats＇ mandible angles, and to assess the value of radionuclide bone imaging in monitoring the effects of this approach. Methods 15 experimental goats were operated with large defects in bilateral mandible angles, and were treated with prefabricated-mineral inducing bone tissue engineered grafts on the left side and without any grafts on the right side（as control group）. Radionuclide bone imaging was used to monitor the effect at the 4 th, 8 th and 12 th postoperative week. Results The 99m^Tc-MDP uptake of the region of interest（ROI） of the control group did not indicate any process of revascularization or bone regeneration. An obvious increasing tendency of the revascularization and bone regeneration was observed in goats of the 8 th postoperative week that received prefabricated-mineral inducing bone tissue engineered grafts. Meanwhile, a slight decreasing tendency of the 99m^Tc-MDP uptake of the region of interest（ROI） was observed in goats at the 12 th week after operation due to the maturation of bone regeneration. Conclusions The prefabricated-mineral inducing bone tissue engineered grafts is eligible in repairing large defects in the goats＇ mandible angles, and radionuclide bone imaging may accurately monitor the revascularization and bone regeneration after the bone grafts implantation.

关 键 词：无机诱导因子 组织工程 放射性核素 

分 类 号：R445.5[医药卫生—影像医学与核医学]