农药杀螟硫磷酶促降解的研究  

Enzymatic Degradation of Fenitrothion

作　　者：张忠辉[1] 洪青[1] 武俊[1] 汪婷[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,南京210095

出　　处：《应用与环境生物学报》2006年第3期395-398,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家863资助项目(2004AA246070;2004214102);国家自然科学基金资助项目(30400013;40471073);江苏省科技攻关资助项目(BE2002345;BE2003343)~~

摘　　要：从长期受农药杀螟硫磷污染的土壤中分离到一株高效降解菌株,研究了其最适产酶条件:培养温度为30℃,培养液起始pH为7.0,培养时间为30 h.从该降解菌中提取的粗酶液在pH 7.5和30℃时显示最大的降解活性,其米氏常数Km为2.92×10-4mol/L,最大降解速率为2 422.79 nmolm in-1mg-1.A bacterium capable of utilizing fenitrothion （ O, O-dimethyl-O-4-nitro-m-tolyl phosphorothioate） as a sole carbon source was isolated from fenitrothion-exposed soil. Its most suitable enzyme production conditions were ： 30℃ , initial pH 7.0 and incubation time 30 h. The crude enzyme extracted from the strain showed the greatest activity was obtained at pH 7.5 and 30 ℃ , with its Michaelis-Mentn＇s constant （Km） and maximum degradation rate of 2.92 × 10^-4 mol/L and 2 422.79 nmol min^-1 mg^-1 , respectively. Fig 7, Ref 15

关 键 词：杀螟硫磷 酶促降解 产酶条件 酶的性质 

分 类 号：X592[环境科学与工程—环境工程]