实验感染HEV IV型后恒河猴HEVRNA及抗HEVIgM(IgG)动态变化研究  

Dynamic changes of HEVRNA and Anti-HEV IgM (IgG) in China Rhesus infected by HEV-IV

作　　者：杨志国[1] 孟继鸿[2] 丁福[2] 鞠九龙[1] 许远红[1] 许家璋[1] 

机构地区：[1]中国人民解放军第81医院南京军区肝病研究所,江苏南京210002 [2]南京东南大学基础医学院,江苏南京210008

出　　处：《中国现代医学杂志》2006年第12期1828-1830,共3页China Journal of Modern Medicine

摘　　要：目的观察实验感染HEV(HepatitisEVirus)IV型后恒河猴HEVRNA及抗HEV-IgM(IgG)的动态变化,探索HEVIV型感染的规律。方法用逆转录PCR检测实验动物接种前后血清及粪便HEVR-NA,用酶联免疫法(ELISA)检测血清抗HEV-IgM及抗HEV-IgG,并同步观察其血生化及肝脏组织学的变化。结果感染后的恒河猴均发生了典型的急性肝炎,血清HEVRNA首先呈现阳性,相继出现谷丙转氨酶(ALT)升高、抗HEV-IgM阳性及抗HEV-IgG阳性。结论中国恒河猴是HEVIV型感染的较好的动物模型,检验资料为阐明HEVIV型感染的规律提供了证据。[Objective] To observe the dynamic changes of HEVRNA and Anti-HEV IgM （IgG） in China Rhesus infected by HEV-Ⅳ. To study the rules of infection of IEV-Ⅳ. [Method] A specific RT-PCR assay was used for the detection of HEV-RNA in sera and stool suspensions of experimentally infected China Rhesus; ELISA was used for the detection of anti-HEV IgM and IgG in sera. The changes of sera biochemistry and liver histopathology were observed synchronizedly. [Result] The two experimental animals got typical acute hepatitis profile. [Conclusion] China Rhesus was a good experiental animal model of HEV-Ⅳ. The result showed solid HEV infection evidence of typical hepatitis after the infection.

关 键 词：戊型肝炎病毒 Ⅳ型 HEVRNA 抗HEV-IGM 抗HEV-IGG 

分 类 号：R332[医药卫生—人体生理学]