Cajal间质细胞和P物质在糖尿病大鼠结肠组织中的改变  

The quantity study of interstitial cells of Cajal and substance P in colon tissue of diabetic rats with gastrointestinal dysfunction using immunohistochemical stain

作　　者：许新芳[1] 林琳[1] 陈娟[1] 张红杰[1] 

机构地区：[1]南京医科大学第一附属医院消化科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第7期555-557,F0002,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金资助(BK2004158);江苏省"135工程"重点人才基金资助(RC2003087)

摘　　要：目的:分析糖尿病大鼠结肠组织中Cajal间质细胞和P物质的分布及表达变化,探讨Cajal间质细胞和P物质在糖尿病结肠功能紊乱发病中的作用。方法:建立糖尿病大鼠模型,免疫组化检测Cajal间质细胞和P物质在糖尿病大鼠结肠组织内的分布和表达。结果:Cajal间质细胞和P物质主要分布在大鼠近端和远端结肠的肌间神经丛,糖尿病大鼠结肠组织内Cajal间质细胞和P物质的表达较正常大鼠均明显减少(P<0.05)。结论:糖尿病大鼠结肠组织内Cajal间质细胞和P物质表达减少,推测与糖尿病大鼠结肠功能紊乱有一定相关性。Objective： To investigate the changes of interstitial cells of Cajal and the expression of substance P in colon tissue of diabetic rats with gastrointestinal dysfunction and to discuss the possible mechanism of gastrointestinal dysfunction associated with diabetes mellitus. Methods： Thirty SD rats were randomly divided into normal control group （n = 10） and diabetic model group（n = 20）. Rats in diabetic model group were made into diabetic models with intraperitoneal injection of streptozotocin（STZ）（80 mg/kg） for only one time. and those in normal control group were intraperitoneally injected with the equal Citric Acid buffer. The distribution of interstitial cells of Cajal and the expression of substance P in the proximal and distal colon tissue were detected by immunohistochemistry. Results： The immunoreaction positive products of interstitial cells of Cajal and substance P were mainly distributed in the myenteric plexus of the colon tissue, and the expression of interstitial cells of Cajal and substance P in the proximal and distal colon tissue of diabetic rats were decreased （P 〈 0.05）. Conclusion： Decreased expression of interstitial cells of Cajal and substance P in the colon tissue might play an important role in the gastrointestinal dysfunction associated with diabetic mellitus.

关 键 词：糖尿病 CAJAL间质细胞 P物质 胃肠功能紊乱 

分 类 号：R587.1[医药卫生—内分泌]