加味丹参饮对大鼠实验性心肌缺血损伤的影响  

Effect of Danshen Yin on Experimental Myocardial Ischemia in Rats

作　　者：廖菁[1] 黄政德[1] 葛金文[1] 喻嵘[1] 邓奕辉[1] 

机构地区：[1]湖南中医药大学,湖南长沙410007

出　　处：《湖南中医学院学报》2006年第3期6-8,共3页Journal of Hunan College of Traditional Chinese Medicine

基　　金：湖南省自然科学基金资助课题(01JJY2039)。

摘　　要：目的研究加味丹参饮对大鼠实验性心肌缺血损伤的影响及部分作用机制。方法采用结扎冠状动脉左前降支的大鼠心肌缺血模型,观察加味丹参饮高、中、低剂量(8.24g/kg、4.12g/kg、2.06g/kg)对造模后大鼠心电图ST段心肌组织的损伤情况以及血清CK、AST、LDH水平、血浆中MDA含量和SOD、GSH-Px活性的影响。结果与造模组比较加味丹参饮高剂量组Ⅱ导联心电图ST段升高幅度明显下降(P<0.05);各加味丹参饮组对AST、LDH、MDA升高和SOD活性下降均有显著的抑制作用(P<0.01),且高剂量组对血清CK的升高、GSH-Px活性下降亦有显著的抑制作用(P<0.05);光镜观察显示加味丹参饮可明显改善心肌结构的损伤。结论加味丹参饮可明显减轻大鼠心肌缺血损伤程度,对实验性心肌缺血具有显著的保护作用,这与其抗过氧化作用密切相关。Objective To study the effect and mechanism of Supplemented Danshen Yin （DSY） on myocardial ischemia in rats. Methods Myocardial ischemia of rat models were induced by occluding the left anterior descending coronary artery, the effects of DSY （8.24,4.12, 2.06 g/kg） on the creatine kinase （CK）, aspartate aminotransferase （AST）, lactate dehydrogenase （LDH）, malondialdehyde （MDA）, super oxide dismutase （SOD） and glutathione-px （GSH- Px） contents in rats were observed. Results Compared with model group, the raise of ST segment in the standard lead Ⅱ of ECG in high dosage DSY group obviously decreased （P〈0.05）. And DSY in all dosage could decrease AST, LDH and MDA contents, and increase the activity of SOD （P〈0.01） as well Meanwhile, the group with the high dosage could decrease CK contents and increase the activity of GSH-Px （P〈0.05）. And DSY could improve obviously the injury in the heart structure with the light microscope. Conclusion DSY has protective effects to improve the experimental myocardial ischemia in rats,which is closely related to anti-peroxidation.

关 键 词：加味丹参饮 心肌缺血损伤保护作用 抗过氧化 大鼠 丹参 檀香 

分 类 号：R285.5[医药卫生—中药学]