补阳还五汤对脑出血大鼠脑内Caspase-3活化的影响  

Effect of Buyanhuanwu Decoction on Activated Caspase-3 after Intracerebral Hemorrhage in Rats

作　　者：罗杰坤[1] 唐涛[1] 黎杏群[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《湖南中医学院学报》2006年第3期12-14,共3页Journal of Hunan College of Traditional Chinese Medicine

基　　金：湖南省自然科学基金(03JJY3048)。

摘　　要：目的研究补阳还五汤对脑出血大鼠的保护作用。方法采用微量注射器向大鼠苍白球内注入Ⅶ型胶原酶0.4U建立脑出血模型,用免疫组织化学法观察脑出血后1、2、4、7d,4个时间点活化Caspase-3表达变化,以阳性细胞计数作为观察指标,并观察血肿大小的变化。结果脑出血后1d大鼠血肿周围活化Caspase-3阳性神经元细胞计数最多,2d开始下降,7d明显下降,与假手术组比较具有统计学意义(P<0.01);补阳还五汤组与同时间点模型组比较:1、2d计数无统计学意义,4、7d均明显减少(P<0.05)。造模后1d血肿最大,2d有所变小,7d明显变小;治疗组在1、2d与模型组比较血肿大小无明显差异(P>0.05),4、7d具统计学意义(P<0.05)。结论大鼠脑出血后血肿周围神经元活化Caspase-3表达明显上调;补阳还五汤能下调Caspase-3的活化,减少神经元的凋亡,同时促进血肿的吸收。Objective To observe the protective effect of Buyanhuanwu decoction （BHD） after intracerebral hemorrhage（ICH） in rats. Methods The ICH model was made with injecting Ⅶ type collagenase stereotaxically into the globas pallidus of rats. The expression of activated Caspase-3 was examined with Immunohistochemical methods, and the degree of the hematoma was measured at different time. Results The peak value of positive cells around the hematoma was the most at 1^st day, then descended at 24 days and descended distinctly at 7^th days compared with the fake group, which was different evidently（P〈0.01）. The number of positive cells of the BHO group was obviously smaller than that of the model group at 4^th d.7^th d （P〈0.05）, the hematoma was the biggest at 1^st d, smaller at 2^nd d and the smallest at 7^th d. The hematoma in treatment group became smaller obviously than that did in model group at 4^th d and 7^th d （P〈 0.05）. Conclusion The expression of activated Caspase-3 increased obviously in the neuron around the hematoma after ICH, but BHD can markedly decrease the expression of activated Caspase-3, prohibit neuronal apeptosis, and promote the absorption of the hematoma.

关 键 词：脑出血 补阳还五汤 CASPASE-3 大鼠 黄芪 当归 地龙 川芎 

分 类 号：R285.5[医药卫生—中药学]